Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2021 Jun 28;21(1):133.
doi: 10.1186/s12902-021-00795-6.

Nicotinamide attenuates streptozotocin-induced diabetes complications and increases survival rate in rats: role of autonomic nervous system

Paula L Cruz  1 , Ivana C Moraes-Silva  1 , Amanda A Ribeiro  1 , Jacqueline F Machi  1 , Marcelo Dantas Tavares de Melo  1 , Fernando Dos Santos  1 , Maikon Barbosa da Silva  1 , Celia Maria Cassaro Strunz  1 , Elia Garcia Caldini  2 , Maria-Claudia Irigoyen  3
Affiliations

Nicotinamide attenuates streptozotocin-induced diabetes complications and increases survival rate in rats: role of autonomic nervous system

Paula L Cruz et al. BMC Endocr Disord. .

Abstract

Background: To evaluate the effect of nicotinamide prior to streptozotocin-induced (STZ) diabetes in baroreflex sensitivity and cardiovascular autonomic modulation, and its association with hemodynamics and metabolic parameters.

Methods: Methods: Male Wistar rats were divided into control (Cont) and STZ-induced diabetes (Diab). Half of the rats from each group received a single dose of nicotinamide (100 mg/Kg) before STZ injection (Cont+NicA and Diab+NicA). All groups were followed-up for 5 weeks.

Results: Body weight loss of more than 40% was observed in Diab throughout the period (Diab: 271.00 ± 12.74 g; Diab+NicA: 344.62 ± 17.82). Increased glycemia was seen in Diab rats (541.28 ± 18.68 mg/dl) while Diab+NicA group had a slight decrease (440.87 ± 20.96 mg/dl). However, insulin resistance was observed only in Diab. In relation to Cont, heart rate, mean blood pressure and diastolic function were reduced when compared to Diab, together with parasympathetic modulation and baroreflex sensitivity. All of these parameters were improved in Diab+NicA when compared to Diab. Improved baroreflex sensitivity and parasympathetic modulation were correlated with glycemia, insulin resistance, and body weight mass. Additionally, Diab+NicA group increased survival rate.

Conclusions: Results suggest that the association of nicotinamide in STZ-induced diabetic rats prevents most of the expected derangements mainly by preserving parasympathetic and baroreflex parameters.

Keywords: Autonomic nervous system; Diabetes; Nicotinamide.

PubMed Disclaimer

Conflict of interest statement

The authors declare that they have no competing interests.

Figures

Fig. 1
Fig. 1
1A, Body weight of control (Cont, n = 8); control nicotinamide (Cont+NicA, n = 7); diabetes (Diab, n = 7) and diabetes nicotinamide (Diab+NicA, n = 8) groups; *p ≤ 0.0001 vs. Cont; #p ≤ 0.0001 vs. Diab; †p ≤ 0.0001 vs. Cont+NicA; ‡p ≤ 0.0001 week 5 vs. week 0 in the same group. 1B, Lee index of control rats (Cont, n = 8); control nicotinamide (Cont+NicA, n = 7); diabetes (Diab, n = 7) and diabetes nicotinamide (Diab+NicA, n = 8); *p ≤ 0.0001 vs. Cont; #p ≤ 0.0001 vs. Diab
Fig. 2
Fig. 2
2A, Fasting glycemia of control (Cont, n = 8); control nicotinamide (Cont+NicA, n = 7); diabetes (Diab, n = 7) and diabetes nicotinamide (Diab+NicA, n = 8) groups; *p ≤ 0.0001 vs. Cont; †p ≤ 0.0001 vs. Cont+NicA; ‡p ≤ 0.0001 week 5 vs. week 0 in the same group. 2B, Insulin tolerance test evaluated by the constant decrease for plasma glucose (kITT) of control rats (Cont, n = 8) control nicotinamide (Cont+NicA, n = 7); diabetes (Diab, n = 7) and diabetes nicotinamide (Diab+NicA, n = 8); *p ≤ 0.0001 vs. Cont; #p ≤ 0.0001 vs. Diab. 2C, Fasting triglycerides levels of control rats (Cont, n = 8); control nicotinamide (Cont+NicA, n = 7); diabetes (Diab, n = 7) and diabetes nicotinamide (Diab+NicA, n = 8); *p ≤ 0.05 vs. Cont; #p ≤ 0.01 vs. Diab
Fig. 3
Fig. 3
Baroreceptor reflex sensitivity evaluated by bradicardiac and tachycardic responses of control (Cont, n = 8); control nicotinamide (Cont+NicA, n = 7); diabetes (Diab, n = 7) and diabetes nicotinamide (Diab+NicA, n = 8) groups; *p ≤ 0.05 vs. Cont; #p ≤ 0.05 vs. Diab; †p ≤ 0.05 vs. Cont+NicA
Fig. 4
Fig. 4
Survival percentage estimated by the Kaplan-Meier method of control (Cont, n = 8); control nicotinamide (Cont+NicA, n = 7); diabetes (Diab, n = 7) and diabetes nicotinamide (Diab+NicA, n = 8) groups; ¥ ≤ 0.05 vs. all groups
See this image and copyright information in PMC

References

    1. Association A Classification and diagnosis of diabetes. Diabetes care. 2017;40(Supplement 1):S11–S24. doi: 10.2337/dc17-S005. - DOI - PubMed
    1. Chen C, Cohrs CM, Stertmann J, Bozsak R, Speier S. Human beta cell mass and function in diabetes: recent advances in knowledge and technologies to understand disease pathogenesis. Mol Metab. 2017;6(9):943–957. doi: 10.1016/j.molmet.2017.06.019. - DOI - PMC - PubMed
    1. Barcala Tabarrozzi AE, Castro CN, Dewey R, Sogayar M, Labriola L, Perone MJ. Cell-based interventions to halt autoimmunity in type 1 diabetes mellitus. Clin Exp Immunol. 2013;171(2):135–146. doi: 10.1111/cei.12019. - DOI - PMC - PubMed
    1. Hansen JB, Arkhammar G, Per O, Bodvarsdottir TB, Wahl P. Inhibition of insulin secretion as a new drug target in the treatment of metabolic disorders. Curr Med Chem. 2004;11(12):1595–1615. doi: 10.2174/0929867043365026. - DOI - PubMed
    1. Lencioni C, Lupi R, Del Prato S. Beta-cell failure in type 2 diabetes mellitus. Curr Diab Rep. 2008;8(3):179–184. doi: 10.1007/s11892-008-0031-0. - DOI - PubMed

MeSH terms

LinkOut - more resources