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. 2021 May 21:42:100906.
doi: 10.1016/j.nmni.2021.100906. eCollection 2021 Jul.

Taxonogenomics description of Bacillus marasmi sp. nov., a new species isolated from the stool sample

M Sarr  1   2 , F S Diouf  1   2 , C I Lo  2   3 , M Tidjani Alou  1   2 , S Alibar  1   2 , M Million  1   2 , C Sokhna  2   4 , F Fenollar  2   3
Affiliations

Taxonogenomics description of Bacillus marasmi sp. nov., a new species isolated from the stool sample

M Sarr et al. New Microbes New Infect. .

Abstract

Using the culturomics method, two strains were isolated, identified, and characterised following the taxonogenomics concept. Bacillus marasmi sp. nov. strain Marseille-P3556 (= CSURP3556) is isolated from a 13-month-old girl living in Niger. The phylogenetic tree, phenotypic criteria, and genomic analysis described here clearly show that this bacterium is different from previously known bacterial species withstanding in nomenclature and new members of Bacillus genus.

Keywords: Bacillus marasmi sp. nov.; child; human stool; marasmus; taxonogenomics.

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Figures

Fig. 1
Fig. 1
Matrix-assisted laser desorption ionisation–time-of-flight mass spectrometry (MALDI-TOF MS) reference spectrum of Bacillus marasmi sp. nov. The reference spectrum was generated by comparison of spectra from 12 individual colonies of strain Marseille-P3556.
Fig. 2
Fig. 2
The 16S rRNA phylogenetic tree displaying the position of Bacillus marasmi strain Marseille-P3556T relative to its closest phylogenetically species. The respective GenBank accession numbers for 16S rRNA genes are indicated in parenthesis. Sequence alignment and phylogenetic inferences were obtained using the maximum likelihood method within MEGA 7 software. The numbers at the nodes are percentages of bootstrap values obtained by repeating the analysis 1000 times to generate a majority consensus tree.
Fig. 3
Fig. 3
Scanning electron micrograph of Bacillus marasmi strain Marseille-P3556T using the Scanning Electron Microscope SU5000 from Hitachi. Scale bar and acquisition settings are presented on the pictures.
Fig. 4
Fig. 4
Circular map of the genome Bacillus marasmi strain Marseille-P3556 (4,638,282 bp). CGView Server [34] was used to perform this genome cartography. From outside to the centre: region coding genes and RNA genes from the forward and reverse strands, respectively, GC content (black) and GC skew (green/mauve).
Fig. 5
Fig. 5
Heatmap generated with OrthoANI values calculated using the OAT software for Bacillus marasmi sp. nov., strain Marseille-P3556 with its respective closely related species with standing in nomenclature.
See this image and copyright information in PMC

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