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Review
. 2022 Oct;289(19):5850-5863.
doi: 10.1111/febs.16098. Epub 2021 Jul 12.

Probing invadosomes: technologies for the analysis of invadosomes

Shan-Shan Lin  1 , You-An Su  1 , Mei-Chun Chuang  1 , Ya-Wen Liu  1   2
Affiliations
Free article
Review

Probing invadosomes: technologies for the analysis of invadosomes

Shan-Shan Lin et al. FEBS J. 2022 Oct.
Free article

Abstract

Invadosomes are protrusive and mechanosensitive actin devices critical for cell migration, invasion, and extracellular matrix remodeling. The dynamic, proteolytic, and protrusive natures of invadosomes have made these structures fascinating and attracted many scientists to develop new technologies for their analysis. With these exciting methodologies, many biochemical and biophysical properties of invadosomes have been well characterized and appreciated, and those discoveries elegantly explained the biological and pathological effects of invadosomes in human health and diseases. In this review, we focus on these commonly used or newly developed methods for invadosome analysis and effort to reason some discrepancies among those assays. Finally, we explore the opposite regulatory mechanisms among invadosomes and focal adhesions, another actin-rich adhesive structures, and speculate a potential rule for their switch.

Keywords: focal adhesion; invadosome dynamics; proteolytic activity; protrusive force; stiffness.

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