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. 2021 Jul 1;61(3):91-112.
doi: 10.1097/IIO.0000000000000362.

Manufacturing of Viral Gene Therapies

Manufacturing of Viral Gene Therapies

G Anthony Ramirez et al. Int Ophthalmol Clin. .
No abstract available

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References

    1. Shahryari A, Saghaerian JM, Mohammadi S, et al. Development and clinical translation of approved gene therapy products for genetic disorders. Front Genet. 2019;10:868.
    1. Wright JF. Transient Transfection methods for clinical adeno-associated viral vector production. Hum Gene Ther. 2009;20:698–706.
    1. Xiao X, Li J, Samulski RJ. Production of high-titer recombinant adeno-associated virus vectors in the absence of helper adenovirus. J Virol. 1998;72:2224–2232.
    1. Grimm D, Kay MA, Kleinschmidt JA. Helper virus-free, optically controllable, and two-plasmid-based production of adeno-associated virus vectors of serotypes 1 to 6. Mol Ther. 2003;7:839–850.
    1. Powers AD, Piras BA, Clark RK, et al. Development and optimization of AAV hFIX particles by transient transfection in an iCELLis(®) fixed-bed bioreactor. Hum Gene Ther Methods. 2016;27:112–121.

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