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. 2021 Jun 24;10(7):802.
doi: 10.3390/pathogens10070802.

Whole Genome Sequence Analysis of Mycobacterium bovis Cattle Isolates, Algeria

Fatah Tazerart  1   2   3 , Jamal Saad  3   4 , Naima Sahraoui  2 , Djamel Yala  5 , Abdellatif Niar  6 , Michel Drancourt  3   4
Affiliations

Whole Genome Sequence Analysis of Mycobacterium bovis Cattle Isolates, Algeria

Fatah Tazerart et al. Pathogens. .

Abstract

Mycobacterium bovis (M. bovis), a Mycobacterium tuberculosis complex species responsible for tuberculosis in cattle and zoonotic tuberculosis in humans, is present in Algeria. In Algeria however, the M. bovis population structure is unknown, limiting understanding of the sources and transmission of bovine tuberculosis. In this study, we identified the whole genome sequence (WGS) of 13 M. bovis strains isolated from animals exhibiting lesions compatible with tuberculosis, which were slaughtered and inspected in five slaughterhouses in Algeria. We found that six isolates were grouped together with reference clinical strains of M. bovis genotype-Unknown2. One isolate was related to M. bovis genotype-Unknown7, one isolate was related to M. bovis genotype-Unknown4, three isolates belonged to M. bovis genotype-Europe 2 and there was one new clone for two M. bovis isolates. Two isolates from Blida exhibited no pairwise differences in single nucleotide polymorphisms. None of these 13 isolates were closely related to four zoonotic M. bovis isolates previously characterized in Algeria. In Algeria, the epidemiology of bovine tuberculosis in cattle is partly driven by cross border movements of animals and animal products.

Keywords: Algeria; Mycobacterium bovis; Mycobacterium tuberculosis; cattle; tuberculosis.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Location of the five slaughterhouses in four departments in northern Algeria.
Figure 2
Figure 2
Phylogeny tree of 105 genome sequence of Mycobacterium bovis based on 2881 variable positions extracted using MTBseq. The scale bar indicates the number of substitutions per polymorphic site. The tree was generated using PhyML 3.0 online tools.
See this image and copyright information in PMC

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