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. 2021 Jun 12;9(6):1287.
doi: 10.3390/microorganisms9061287.

Comparative Exoproteome Analysis of Streptococcus suis Human Isolates

Esther Prados de la Torre  1 , Antonio Rodríguez-Franco  1 , Manuel J Rodríguez-Ortega  1
Affiliations

Comparative Exoproteome Analysis of Streptococcus suis Human Isolates

Esther Prados de la Torre et al. Microorganisms. .

Abstract

The swine pathogen Streptococcus suis is a Gram-positive bacterium which causes infections in pigs, with an impact in animal health and in the livestock industry, and it is also an important zoonotic agent. During the infection process, surface and secreted proteins are essential in the interaction between microorganisms and their hosts. Here, we report a comparative proteomic analysis of the proteins released to the extracellular milieu in six human clinical isolates belonging to the highly prevalent and virulent serotype 2. The total secreted content was precipitated and analyzed by GeLC-MS/MS. In the six strains, 144 proteins assigned to each of the categories of extracellular or surface proteins were identified, as well as 680 predicted cytoplasmic proteins, many of which are putative moonlighting proteins. Of the nine predicted signal peptide-I secreted proteins, seven had relevant antigenic potential when they were analyzed through bioinformatic analysis. This is the first work comparing the exoproteome fraction of several human isolates of this important pathogen.

Keywords: Streptococcus suis; human infection; moonlighting proteins; proteomics; secreted proteins; vaccine candidates; zoonosis.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Principal component analysis (PCA) of the proteins identified in the six Streptococcus suis human clinical isolates.
Figure 2
Figure 2
Hierarchically-clustered heatmaps of the z-scored predicted surface protein abundances in the six Streptococcus suis human clinical isolates: (a) lipoproteins, (b) cell wall proteins, (c) secreted proteins, (d) membrane proteins. The proteins are clustered in columns in each heatmap, and the isolates are shown in rows. The numbers in parenthesis in clinical isolates representing each of the three biological replicates.
Figure 2
Figure 2
Hierarchically-clustered heatmaps of the z-scored predicted surface protein abundances in the six Streptococcus suis human clinical isolates: (a) lipoproteins, (b) cell wall proteins, (c) secreted proteins, (d) membrane proteins. The proteins are clustered in columns in each heatmap, and the isolates are shown in rows. The numbers in parenthesis in clinical isolates representing each of the three biological replicates.
See this image and copyright information in PMC

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