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Comment
. 2021 Jun 18;10(6):1534.
doi: 10.3390/cells10061534.

Introduction of Mouse Embryonic Fibroblasts into Early Embryos: From Confusion to Constructive Discussion. Comment on Savatier, P. Introduction of Mouse Embryonic Fibroblasts into Early Embryos Causes Reprogramming and (Con)fusion. Cells 2021, 10, 772

Krystyna Żyżyńska-Galeńska  1 , Jolanta Karasiewicz  1 , Agnieszka Bernat  1   2
Affiliations
Comment

Introduction of Mouse Embryonic Fibroblasts into Early Embryos: From Confusion to Constructive Discussion. Comment on Savatier, P. Introduction of Mouse Embryonic Fibroblasts into Early Embryos Causes Reprogramming and (Con)fusion. Cells 2021, 10, 772

Krystyna Żyżyńska-Galeńska et al. Cells. .

Abstract

We would like to address the issues raised by Pierre Savatier in "Introduction of Mouse Embryonic Fibroblasts into Early Embryos Causes Reprogramming and (Con)Fusion" [...].

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Conflict of interest statement

The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.

Figures

Figure 1
Figure 1
Development to the blastocyst of chimeric embryos carrying MEF cell-expressing RFP (red). (A) Chimeric embryo with MEF cell-expressing RFP (red) being eliminated from the embryo after blastomere puncturing; (B) development of chimeric embryo to blastocyst with MEF cell-expressing RFP (red), integrated with the embryo without blastomere puncturing; (C) immunofluorescent staining of blastocyst with MEF cell expressing RFP (red), integrated with the embryo without blastomere puncturing; (D) RT-PCR analysis of KLF4 expression in MEF cells at passage 2 (p2) and 4 (p4), as compared to mouse embryonic stem cells. Materials and methods are described in [2].
See this image and copyright information in PMC

Comment on

References

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