In Vitro Assessment of Anticoccidials: Methods and Molecules
- PMID: 34209100
- PMCID: PMC8300270
- DOI: 10.3390/ani11071962
In Vitro Assessment of Anticoccidials: Methods and Molecules
Abstract
Avian coccidiosis is a disease causing considerable economic losses in the poultry industry. It is caused by Eimeria spp., protozoan parasites characterized by an exogenous-endogenous lifecycle. In vitro research on these pathogens is very complicated and lacks standardization. This review provides a description of the main in vitro protocols so far assessed focusing on the exogenous phase, with oocyst viability and sporulation assays, and on the endogenous phase, with invasion and developmental assays in cell cultures and in ovo. An overview of these in vitro applications to screen both old and new remedies and to understand the relative mode of action is also discussed.
Keywords: Eimeria; botanicals; cell culture; essential oils; in vitro; invasion assay; poultry.
Conflict of interest statement
Andrea Piva serves as a professor at the University of Bologna and is a member of the board of directors of Vetagro S.p.A. (Reggio Emilia, Italy), which funded the project. Ester Grilli serves as an advisor of Vetagro.
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