Evaluation of antinociceptive activity of Ilex dipyrena Wall. in mice

Abstract

Background: In order to find a new natural resource for pain-relief, the analgesic effects of Ilex dipyrena crude extract, fractions, and subfractions were evaluated in in-vivo mouse models with possible mechanism of action.

Methods: Analgesic effects of crude extract (100 and 200 mg/kg body weight), fractions and subfractions (75 mg/kg body weight) were screened using heat-induced (tail-immersion and hot plate test) and chemical-induced (formalin and acetic acid) nociception models in mice. The samples were also tested for the elucidation of a possible mechanism through opioidergic and GABAergic systems.

Results: The administration of crude extract, fractions and subfractions produced analgesic responses in acetic acid, formalin, tail immersion, and hot plate model for pain similar to those obtained with the standard. Naloxone antagonized the antinociceptive effects of the tested samples, whereas bicuculline showed partial inhibition. Considering the analgesic response, crude extract, fractions, and subfractions demonstrated promising inhibitory activity against all test models for pain, which was further supported by the possible involvement of opioidergic and GABAergic systems.

Conclusion: The results suggest that this plant may be useful in the development of new analgesic drugs. Further research with regard to the isolation of bioactive compounds is required to verify these findings.

Keywords: Analgesic effect; GABAergic; Ilex dipyrena; Mouse models; Opioidergic.

Fig. 1

analgesic activity of I. dipyrena using acetic acid model. Mean ± SEM (n = 8). Where, ^*P < 0.05, ^**P < 0.01 and ^***P < 0.001 statistically significant relative to control

Fig. 2

Formalin-induced licking response in 1st and 2nd Phase. Mean ± SEM (n = 8). Where, ^*P < 0.05, ^**P < 0.01 and ^***P < 0.001 statistically significant relative to control