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. 2021 Jun 29;41(2):260-270.
doi: 10.7705/biomedica.5409.

Seroprevalence of Brucella canis in canines from a dog shelter in Bogotá, Colombia

[Article in English, Spanish]
Astrid-Jullieth Laverde  1 , Daniela Restrepo-Botero  1 , Diego Hernández-Pulido  1 , José Luis Rodríguez-Bautista  2 , Isabel-Sofía Sandoval  3
Affiliations

Seroprevalence of Brucella canis in canines from a dog shelter in Bogotá, Colombia

[Article in English, Spanish]
Astrid-Jullieth Laverde et al. Biomedica. .

Abstract
in English, Spanish

Introduction: The risk of Brucella canis infection in humans and dogs has increased due to the permanent exposure to asymptomatic carrier dogs. In Colombia, there is evidence of B. canis infection in humans living with dogs. In the case of Bogotá, an additional concern is the lack of updated information related to the prevalence of the infection in dogs. Objective: To determine the seroprevalence of infection by B. canis in dogs intended for adoption programs in Bogotá. Materials and methods: By means of a descriptive cross-sectional study carried out in a dog shelter in Bogotá, anti-B. canis IgG antibodies were detected in the serum from 51 dogs 28 females and 23 males) using a lateral-flow immunochromatographic test. Additionally, seropositive animals were analyzed with PCR to detect Brucella spp DNA. Results: Brucella canis seroprevalence was 1.96% (1/51). The seropositive dog was an asymptomatic three-year-old she-dog in which no bacteria DNA was detected in the blood through PCR. Conclusions: The seroprevalence determined in this study represented by a single dog with anti-B. canis IgG can be considered a potential risk both for canine and human populations since this single dog could have a persistent infection capable of spreading the bacteria.

Introducción. El riesgo de infección con Brucella canis en humanos y perros aumenta con la exposición constante a perros portadores asintomáticos. En Colombia hay evidencia de infección con B. canis en personas que conviven con perros. Una preocupación adicional en Bogotá es la falta de información actualizada sobre la prevalencia de la infección en perros destinados a programas de adopción. Objetivo. Establecer la seroprevalencia de la infección por B. canis en perros de un refugio para animales de compañía destinados a la adopción en Bogotá. Materiales y métodos. Se hizo un estudio descriptivo de corte transversal en un refugio ara animales de Bogotá. Se detectaron anticuerpos contra B. canis en el suero de 51 perros (28 hembras y 23 machos) mediante una prueba inmunocromatográfica de flujo lateral. Asimismo, los individuos positivos se analizaron con PCR para la detección del ADN de Brucella spp. Resultado. La seroprevalencia de B. canis fue del 1,96 % (1/51). El perro seropositivo correspondió a una hembra asintomática de tres años de edad en la cual no se detectó ADN bacteriano en sangre mediante la PCR. Conclusiones. La seroprevalencia representada por un solo perro con IgG anti-B. canis puede considerarse un riesgo potencial para las poblaciones de perros y humanos, ya que podría tratarse de un animal con infección persistente capaz de diseminar la bacteria.

Keywords: Brucellosis; dogs; zoonoses; chromatography; affinity; public health.

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Figures

Figura 1
Figura 1. Electroforesis en gel de agarosa al 1 % de la PCR de un fragmento del gen bp26 de Brucella spp. en muestra de sangre completa del perro seropositivo para IgG anti-B. canis. Marcador: marcador de peso molecular (Generuler 50 bp DNA Ladder™); C-: control negativo (agua); C+: control positivo. ADN de una cepa (M-) de B. canis, amplicón de 451 pb, aproximadamente; muestra: muestra de sangre negativa
See this image and copyright information in PMC

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