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. 1988 Feb;170(2):653-6.
doi: 10.1128/jb.170.2.653-656.1988.

Genetic transformation system in the archaebacterium Methanobacterium thermoautotrophicum Marburg

V E Worrell  1 , D P Nagle Jr, D McCarthy, A Eisenbraun
Affiliations

Genetic transformation system in the archaebacterium Methanobacterium thermoautotrophicum Marburg

V E Worrell et al. J Bacteriol. 1988 Feb.

Abstract

A wild-type strain of Methanobacterium thermoautotrophicum Marburg was transformed by DNA from strains resistant to 5-fluorouracil. Recipient cells were grown without selection on gellan gum (GELRITE) plates with DNA. Drug-resistant cells were recovered by replica plating the resulting colonies onto drug plates. Transformation required high-molecular-weight DNA with appropriate markers and was not observed on agar or in liquid media under a variety of conditions.

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References

    1. Appl Environ Microbiol. 1976 Dec;32(6):781-91 - PubMed
    1. Sex Transm Dis. 1986 Jul-Sep;13(3 Suppl):185-8 - PubMed
    1. J Bacteriol. 1985 Apr;162(1):461-2 - PubMed
    1. Appl Environ Microbiol. 1984 Feb;47(2):427-9 - PubMed
    1. Microbiol Rev. 1979 Jun;43(2):260-96 - PubMed

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