Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2020 Jun 30;1(1):30-36.
doi: 10.1016/j.xfre.2020.04.006. eCollection 2020 Jun.

Tobacco consumption is associated with slow-growing day-6 blastocysts

Mathilde Bourdon  1   2 , Lucile Ferreux  3 , Chloé Maignien  1 , Catherine Patrat  3 , Louis Marcellin  1   2 , Khaled Pocate-Cheriet  3 , Charles Chapron  1   2 , Pietro Santulli  1   2
Affiliations

Tobacco consumption is associated with slow-growing day-6 blastocysts

Mathilde Bourdon et al. F S Rep. .

Abstract

Objective: To investigate if there are any obvious clinical factors associated with delayed blastulation at day 6 (D6) compared with day 5 (D5).

Design: Monocentric observational cohort study from November 2012 to December 2018.

Setting: Tertiary-care academic medical center.

Patients: A total of 941 women with an entire cohort of exclusively D5 blastocysts compared with 162 patients with a cohort of exclusively D6 blastocysts.

Interventions: None.

Main outcome measures: Clinical characteristics and data related to the ovarian stimulation protocols.

Results: After univariate analysis, a significantly higher proportion of women who were active smokers was found in the D6 group compared with the D5 group (n = 22/162 [13.6%] vs. n = 82/941 [8.7%]). In addition, the women in the D6 group had a higher rank number of assisted reproductive technology (ART; total no. of ART cycles performed: 2.1 ± 1.4 vs. 1.6 ± 1.1) and a lower antral follicle count (AFC; 18.7 ± 11.3 vs. 22.2 ± 12.8). Moreover, fertilization with the use of intracytoplasmic sperm injection was used more frequently in the D6 group compared with the D5 group. Logistic regression analysis adjusted for confounders highlighted several independent predictors for reaching blastocyst stage at D6 rather than D5: being an active smoker, previous ART cycles, and a lower AFC.

Conclusions: Obtaining an exclusively D6 blastocyst cohort is independently associated with women who are active smokers, previous ART cycles, and a lower AFC. These findings provide evidence, to be confirmed by further studies, that women who are active smokers could greatly benefit from smoking cessation before undergoing ART.

Keywords: Assisted reproductive technology; blastocyst; day 5; day 6.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Patient inclusion flowchart. ICSI = intracytoplasmic sperm injection; IVF = in vitro fertilization.
See this image and copyright information in PMC

References

    1. Gardner D.K. Blastocyst culture: toward single embryo transfers. Hum Fertil (Camb) 2000;3:229–237. - PubMed
    1. Glujovsky D., Farquhar C., Quinteiro Retamar A.M., Alvarez Sedo C.R., Blake D. Cleavage stage versus blastocyst stage embryo transfer in assisted reproductive technology. Cochrane Database Syst Rev. 2016 - PubMed
    1. Gardner D.K., Lane M. Culture and selection of viable blastocysts: a feasible proposition for human IVF? Hum Reprod Update. 1997;3:367–382. - PubMed
    1. Wilson M., Hartke K., Kiehl M., Rodgers J., Brabec C., Lyles R. Integration of blastocyst transfer for all patients. Fertil Steril. 2002;77:693–696. - PubMed
    1. Voelker R. Researchers in Canada call for policy to mandate single-embryo transfer in IVF. JAMA. 2011;305:1848. - PubMed

LinkOut - more resources