Liver alcohol and aldehyde dehydrogenase isoenzymes in Chinese

Abstract

Alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) isoenzyme phenotypes were determined in 71 autopsy liver specimens by agarose isoelectric focusing and starch gel electrophoresis, respectively. Ninety percent of the livers examined exhibited a pH-optimum for ethanol oxidation at pH 8.5, instead of 10.5 which is usual among Caucasians. The homozygous ADH2 2-2 phenotype was found to be 59%, and the heterozygous ADH2 2-1 phenotype, 31%. The remaining 10% of the livers, exhibiting a pH-optimum at 10.5, were homozygous ADH2 1-1 phenotype. Accordingly, the gene frequencies of the alleles ADH2(2) and ADH2(1) were calculated to be 0.75 and 0.25, respectively. Fifty-eight percent of the 71 livers examined possessed both the activities of ALDH I and ALDH II isoenzymes, whereas 42% lacked the ALDH I activity. The implications of these distributions of liver ADH and ALDH isoenzyme patterns among Orientals for ethanol elimination, alcohol sensitivity and alcoholism are discussed.