Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2021:2314:549-577.
doi: 10.1007/978-1-0716-1460-0_24.

Targeted Lipidomics of Mycobacterial Lipids and Glycolipids

Emilie Layre  1
Affiliations

Targeted Lipidomics of Mycobacterial Lipids and Glycolipids

Emilie Layre. Methods Mol Biol. 2021.

Abstract

Decades of study have highlighted the richness and uniqueness of the repertoire of lipid and glycolipid families produced by mycobacteria. Many of these families potently regulate host immune responses, in stimulatory or suppressive ways. Thus, the global study of this repertoire in different genetic backgrounds or under model conditions of infection is gaining interest. Despite the difficulties associated with the specificities of this repertoire, the field of mass spectrometry-based lipidomics of mycobacteria has recently made considerable progress, particularly at the analytical level. There is still considerable scope for further progress, especially with regard to the development of an efficient bioinfomatics pipeline for the analysis of the large datasets generated. This chapter describes an HPLC-MS methodology allowing the simultaneous screening of more than 20 of the lipid families produced by mycobacteria and provides recommendations to analyze the generated data given the state-of-the-art.

Keywords: HPLC-MS; Lipidomics; Mass spectrometry; Mycobacterium.

PubMed Disclaimer

References

    1. Jurowski K, Kochan K, Walczak J, Baranska M, Piekoszewski W, Buszewski B (2017) Analytical techniques in Lipidomics: state of the art. Crit Rev Anal Chem 47(5):418–437. https://doi.org/10.1080/10408347.2017.1310613 - DOI - PubMed
    1. Zullig T, Trotzmuller M, Kofeler HC (2020) Lipidomics from sample preparation to data analysis: a primer. Anal Bioanal Chem 412(10):2191–2209. https://doi.org/10.1007/s00216-019-02241-y - DOI - PubMed
    1. Minnikin DE, Brennan PJ (2020) Lipids of clinically significant mycobacteria. In: Goldfine H (ed) Health Consequences of Microbial Interactions with Hydrocarbons, Oils, and Lipids. Handbook of Hydrocarbon and Lipid Microbiology. Springer, Cham, pp 1–76. https://doi.org/10.1007/978-3-319-72473-7_7-1 - DOI
    1. Becker K, Sander P (2016) Mycobacterium tuberculosis lipoproteins in virulence and immunity—fighting with a double-edged sword. FEBS Lett 590(21):3800–3819. https://doi.org/10.1002/1873-3468.12273 - DOI - PubMed
    1. Neyrolles O, Guilhot C (2011) Recent advances in deciphering the contribution of Mycobacterium tuberculosis lipids to pathogenesis. Tuberculosis 91(3):187–195. https://doi.org/10.1016/j.tube.2011.01.002 - DOI - PubMed

LinkOut - more resources