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. 2021 Jul 8;16(7):e0254233.
doi: 10.1371/journal.pone.0254233. eCollection 2021.

High-resolution genotyping of Lymphogranuloma Venereum (LGV) strains of Chlamydia trachomatis in London using multi-locus VNTR analysis-ompA genotyping (MLVA-ompA)

Chloe Manning  1 , Colette O'Neill  1 , Ian N Clarke  1 , Monica Rebec  2 , Penelope R Cliff  3 , Peter Marsh  4
Affiliations

High-resolution genotyping of Lymphogranuloma Venereum (LGV) strains of Chlamydia trachomatis in London using multi-locus VNTR analysis-ompA genotyping (MLVA-ompA)

Chloe Manning et al. PLoS One. .

Abstract

Background: Lymphogranuloma venereum (LGV) is caused by Chlamydia trachomatis strains with ompA genotypes L1 to L3. An LGV epidemic associated with the L2b genotype has emerged in the past few decades amongst men who have sex with men (MSM). C. trachomatis genotypes can be discriminated by outer membrane protein A gene (ompA) sequencing, however this method has limited resolution. This study employed a high-resolution genotyping method, namely, multi-locus tandem repeat (VNTR) analysis with ompA sequencing (MLVA-ompA), to assess the distribution of LGV MLVA-ompA genotypes amongst individuals attending genitourinary medicine (GUM) clinics in London.

Methods: Clinical specimens were collected from individuals attending eight London-based GUM clinics. Specimens that tested positive for C. trachomatis by commercial nucleic acid amplification test (NAAT) were confirmed as LGV by pmpH real-time PCR. LGV-positive DNA extracts were subsequently genotyped using MLVA-ompA.

Results: Two hundred and thirty DNA extracts were confirmed as LGV, and 162 (70%) yielded complete MLVA-ompA genotypes. Six LGV MLVA-ompA genotypes were identified: 1.9.2b-L2, 1.9.3b-L2b, 1.9.2b-L2b, 1.9.2b-L2b/D, 1.4a.2b-L2b, and 5.9.2b-L1. The following LGV ompA genotypes were identified (in descending order of abundance): L2, L2b, L2b/D, and L1. Eight ompA sequences with the hybrid L2b/D profile were detected. The hybrid sequence was identical to the ompA of a recombinant L2b/D strain detected in Portugal in 2017.

Conclusions: The L2 ompA genotype was found to predominate in the London study population. The study detected an unusual hybrid L2b/D ompA profile that was previously reported in Portugal. We recommend further monitoring and surveillance of LGV strains within the UK population.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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References

    1. de Vries HJC, de Barbeyrac B, de Vrieze NHN, Viset J., White JA, Vall-Mayans M, et al.. 2019 European guideline on the management of lymphogranuloma venereum. J Eur Acad Dermatology Venereol. 2019. Oct 26;33(10):1821–8. Available from: https://onlinelibrary.wiley.com/doi/abs/10.1111/jdv.15729 - DOI - PubMed
    1. Götz HM, Ossewaarde JM, Nieuwenhuis RF, van der Meijden WI, Dees J, Thio B, et al.. [A cluster of lymphogranuloma venereum among homosexual men in Rotterdam with implications for other countries in Western Europe]. Ned Tijdschr Geneeskd. 2004. Feb 28;148(9):441–2. Available from: http://www.ncbi.nlm.nih.gov/pubmed/15038207 - PubMed
    1. Spaargaren J, Fennema HSA, Morré SA, de Vries HJC, Coutinho RA. New lymphogranuloma venereum Chlamydia trachomatis variant, Amsterdam. Emerg Infect Dis. 2005. Jul;11(7):1090–2. Available from: http://www.ncbi.nlm.nih.gov/pubmed/16022786 doi: 10.3201/eid1107.040883 - DOI - PMC - PubMed
    1. Spaargaren J, Schachter J, Moncada J, de Vries HJC, Fennema HSA, Peña AS, et al.. Slow Epidemic of Lymphogranuloma Venereum L2b Strain. Emerg Infect Dis. 2005. Nov;11(11):1787–8. Available from: http://wwwnc.cdc.gov/eid/article/11/11/05-0821_article.htm - PMC - PubMed
    1. Isaksson J, Carlsson O, Airell Å, Strömdahl S, Bratt G, Herrmann B. Lymphogranuloma venereum rates increased and Chlamydia trachomatis genotypes changed among men who have sex with men in Sweden 2004–2016. J Med Microbiol. 2017. Nov;66(11):1684–7. Available from: http://www.ncbi.nlm.nih.gov/pubmed/29022544 doi: 10.1099/jmm.0.000597 - DOI - PubMed

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