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. 1978 Jan;34(1):137-47.

Isolation and partial characterization of an antibody enhancing factor from leukaemic owl monkey cell cultures

Isolation and partial characterization of an antibody enhancing factor from leukaemic owl monkey cell cultures

A B MacDonald et al. Immunology. 1978 Jan.

Abstract

Herpesvirus saimiri inoculated into owl monkeys (Aotus trivirgatus) causes leukaemia and lymphoma. Peripheral lymphocytes from leukaemic monkeys grown in culture are predominantly T lymphocytes. The supernatants from these cultures contain a factor which enhances the antibody response of murine B cells to sheep red blood cells (SRBC). The factor has been partially characterized by ammonium sulphate precipitation, DEAE-chromatography, gel filtration over Sephadex G-150 and disc-gel electrophoresis. The enhancing factor is a protein with a molecular weight of approximately 40,000.

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