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. 2022 Jan 18;225(2):243-247.
doi: 10.1093/infdis/jiab350.

Deep Sequencing to Detect Diversity of Trypanosoma cruzi Infection in Patients Coinfected With Human Immunodeficiency Virus and Chagas Disease

Natalie M Bowman  1 , Sujata Balasubramanian  2 , Robert H Gilman  3 , Christian Parobek  2 , Maritza Calderon  4 , Andreea Waltmann  5 , Louisa A Messenger  6 , Leny Sanchez  4 , Caryn Bern  7 , Jonathan J Juliano  1 ; Working Group on Chagas Disease in Bolivia and Peru
Collaborators, Affiliations

Deep Sequencing to Detect Diversity of Trypanosoma cruzi Infection in Patients Coinfected With Human Immunodeficiency Virus and Chagas Disease

Natalie M Bowman et al. J Infect Dis. .

Abstract

Chagas disease, caused by Trypanosoma cruzi, can reactivate and cause severe acute disease in immunocompromised patients such as those infected with human immunodeficiency virus (HIV). We conducted amplicon deep sequencing of a 327-bp fragment of the tcscd5 gene using an Ion Torrent PGM directly from clinical samples from HIV patients with high parasitemia. We describe the within-host diversity, both characterizing the discrete typing unit of the infections and confirming the presence of multistrain infections, directly from clinical samples. This method can rapidly provide information on the genetic diversity of T. cruzi infection, which can have direct impacts on clinical disease.

Keywords: Trypanosoma cruzi; tcsc5d; Chagas disease; HIV; amplicon deep sequencing; discrete typing unit; genetic diversity; multiclonality.

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Figures

Figure 1.
Figure 1.
A, tcsc5d haplotype distribution in human clinical samples, cultured reference strains, and a dog from rural Bolivia. B, Heatmap showing the number of single-nucleotide polymorphism differences (blue and pink) and percentage sequence identity (red and orange) between haplotypes.
See this image and copyright information in PMC

References

    1. World Health Organization. Chagas disease in Latin America: an epidemiological update based on 2010 estimates. Wkly Epidemiol Rec 2015; 90:33–44. - PubMed
    1. Messenger LA, Miles MA, Bern C. Between a bug and a hard place: Trypanosoma cruzi genetic diversity and the clinical outcomes of Chagas disease. Expert Rev Anti Infect Ther 2015; 13:995–1029. - PMC - PubMed
    1. Brenière SF, Waleckx E, Barnabé C. Over six thousand Trypanosoma cruzi strains classified into discrete typing units (DTUs): attempt at an inventory. PLoS Negl Trop Dis 2016; 10:e0004792. - PMC - PubMed
    1. de Almeida EA, Ramos Júnior AN, Correia D, Shikanai-Yasuda MA. Co-infection Trypanosoma cruzi/HIV: systematic review (1980–2010). Rev Soc Bras Med Trop 2011; 44:762–70. - PubMed
    1. Samuels AM, Clark EH, Galdos-Cardenas G, et al. ; Working Group on Chagas Disease in Bolivia and Peru . Epidemiology of and impact of insecticide spraying on Chagas disease in communities in the Bolivian Chaco. PLoS Negl Trop Dis 2013; 7:e2358. - PMC - PubMed

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