Open-source all-in-one LabToGo Office Chromatography

Abstract

Print and media technologies were used uncommonly in the field of chromatography and explored in application to create a miniaturized all-in-one LabToGo system. This novel research field termed Office Chromatography (OC) uses additive manufacturing in terms of 3D printing of operational parts as well as open-source hard- and software. The OCLab2 presented here has been considerably extended in its functionalities. For inkjet printing of solutions, a newly designed printhead was manufactured controlled by a self-constructed ink-jet board, allowing to check the nozzles' resistance heating circuit. Plate heating was newly integrated, especially favorable for the demonstrated application of higher volumes of aqueous samples. The UV/Vis/FLD plate images were captured by a Raspberry Pi V2 camera module under illumination by novel light emitting diodes (LEDs) for highly selective RGBW color (Vis), UVC 278-nm (UV) and UVA 366-nm (FLD) detection, installed in a newly created miniature cabinet to protect from extraneous light. The spectral separation of differently colored food dyes was achieved by the fully addressable driver controlled RGBW LEDs. The software was newly written in R to speed-up the processes, supported by the new Raspberry Pi 4B computer with 4 GB RAM. The analysis of Stevia leaves for steviol glycosides yielded results comparable to the status quo. Different water samples were analyzed for bioactive compounds. Thereby, compounds of general cytotoxicity were effect-directed detected by bioluminescent A. fischeri bacteria. It allowed the bioanalytical screening for potential risks in tap water, surface waters, rain water, landfill leachates and biogas slurries.

Keywords: Cytotoxicity detection; Miniaturized planar chromatography system; On-site analysis; Point-of-care analysis; Stevia leave analysis; Water screening.