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. 2021 Aug;120(8):3001-3005.
doi: 10.1007/s00436-021-07219-3. Epub 2021 Jul 12.

High oxygen concentrations inhibit Acanthamoeba spp

Ines Sifaoui  1   2   3 , Eulalia Capote -Yanes #  4   5 , María Reyes-Batlle  4   6   7 , Rubén L Rodríguez-Expósito  4   6   7 , Isabel L Bazzocchi  8 , Ignacio A Jiménez  8 , José E Piñero  9   10   11 , Jacob Lorenzo-Morales  12   13   14 , Lindell K Weaver  15   16
Affiliations

High oxygen concentrations inhibit Acanthamoeba spp

Ines Sifaoui et al. Parasitol Res. 2021 Aug.

Abstract

Efficacious treatments against Acanthamoeba Keratitis (AK) is challenging, often ineffective and linked to the intragenotype variation in the drug efficacy. Increased oxygen can facilitate host response and can inhibit some organisms. Herein, we report the effect of increased oxygen concentrations on Acanthamoeba spp. growth and its effect on ROS (reactive oxygen species) production. The exposition to pure oxygen could reduce cell growth by at least 60% for Acanthamoeba castellanii Neff, Acanthamoeba polyphaga, and Acanthamoeba griffini. The increase in ROS production confirming that oxygen cell's growth inhibition was due to oxidative stress. Further studies are needed to determine oxygen saturation level, time of oxygen exposition, and number of sessions needed to eliminate the parasite.

Keywords: Acanthamoeba spp; Oxygen therapy; Reactive oxygen species; Viability.

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References

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