Effects of dietary supplementation with Allium mongolicum Regel extracts on growth performance, serum metabolites, immune responses, antioxidant status, and meat quality of lambs

Abstract

The present study was to evaluate the effects of dried Allium mongolicum Regel (AMR) powder and its water- and fat-soluble extracts (AWE and AFE) on the growth performance, serum metabolites, immune responses, antioxidant status, and meat quality of lambs. A total of 32 male small-tailed Han lambs (5 months old; initial body weight = 34.8 ± 0.40 kg) were used in a 60-d feeding experiment after a 15-d adaptation period. The lambs were randomly divided into 4 groups (n = 8) and fed a basal diet (control, CON group), the basal diet supplemented with dried AMR powder at 10 g/d per lamb (AMR group), the basal diet supplemented with AWE at 3.4 g/d per lamb (AWE group), or the basal diet supplemented with AFE at 2.8 g/d per lamb (AFE group). Blood samples were collected on d 0, 30, and 60 in the feeding experiment (n = 8). At the end of the experiment, the lambs were sacrificed and the longissimus dorsi muscles collected. Growth performance was not significantly affected by dietary supplementation of AMR, AWE and AFE (P > 0.05). However, significantly lower albumin (P = 0.006), total protein (P = 0.006), globin (P = 0.025), and blood urea nitrogen (P = 0.024) concentrations were observed in AFE group relative to CON and AMR groups. Similarly, a significantly lower lactate dehydrogenase activity (P = 0.018) was observed in AFE group relative to AWE group, but not in other groups (P > 0.05). In addition, significantly increasing trends in glutathione peroxidase (P = 0.06) in AMR, AWE, and AFE groups were observed relative to the control group. Furthermore, significantly lower drip loss (P = 0.011) across the treatment groups and cooking loss (P = 0.048) were observed in the AMR group relative to the control group. Taken together, these results indicate that AMR and its extracts had no significant effect on lamb growth performance, antioxidant status, and immune responses, but could significantly improve meat quality without the occurrence of pathological kidney and liver lesions.

Keywords: Allium mongolicum Regel; Antioxidant status; Growth performance; Immune response; Meat quality; Serum metabolite.

Figure

Extraction processes for AWE and AFE. ◇, extraction processes; △, solvents. ¹ AMR is the dried leaf powder of Allium mongolicum Regel; ² DDP is decolored and degreased powder; ³ AWEFWR is AMR water-soluble extract fluid with residues; ⁴ AFEFWR is AMR fat-soluble extract fluid with residues; ⁵ AWEF is AWE fluid; ⁶ AFEF is AFE fluid; ⁷ AWE = AMR water-soluble extract; ⁸ AFE = AMR fat-soluble extract. ^a The ratio of AMR to petroleum ether is 1:5 (wt/vol). ^b The ratio of DDP to water is 1:20 (wt/vol). ^c Alcohol 75%; the ratio of DDP to alcohol is 1:30 (wt/vol). The green-labeled matter was thrown away.