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. 1988 Jul;3(1):12.3.1-12.3.6.
doi: 10.1002/j.1934-3647.1988.tb00178.x.

Methylation Interference Assay for Analysis of DNA-Protein Interactions: DNA-Protein Interactions

Albert S Baldwin Jr  1
Affiliations

Methylation Interference Assay for Analysis of DNA-Protein Interactions: DNA-Protein Interactions

Albert S Baldwin Jr. Curr Protoc Mol Biol. 1988 Jul.
No abstract available

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References

Literature Cited
    1. Baldwin, A. and Sharp, P. 1988. Two transcription factors, H2TF1 and NF-kB, interact with a single regulatory sequence in the class I MHC promoter. Proc. Natl. Acad. Sci. U.S.A. 85:723-727.
    1. Brunelle, A. and Schleif, R. 1987. Missing contact probing of DNA-protein interactions. Proc. Natl. Acad. Sci. U.S.A. 84:6673-6676.
    1. Maxam, A. and Gilbert, W. 1980. Sequencing end-labeled DNA with base-specific chemical cleavages. Meth. Enzymol. 65:499-560.
    1. Siebenlist, U. and Gilbert, W. 1980. Contacts between E. coli RNA polymerase and an early promoter of phage T7. Proc. Natl. Acad. Sci. U.S.A. 77:122-126.
    1. Singh, H., LeBowitz, J., Baldwin, A., and Sharp, P.A. 1988. Molecular cloning of an enhancer binding protein: Isolation by screening of an expression library with a recognition site DNA. Cell 52:415-423.
Key References
    1. Hendrickson, W. and Schleif, R. 1985. A dimer of AraC protein contacts three adjacent major groove regions at the Ara I DNA site. Proc. Natl. Acad. Sci. U.S.A. 82:3129-3133.
    1. Provides good descriptions of the methylation interference procedure as well as ethylation interference. Furthermore, interpretations of major groove contacts are described.
    1. Maxam and Gilbert, 1980. See above.
    1. Describes all of the basics for labeling DNA, modification by DMS, and polyacrylamide gel electrophoresis.

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