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Review
. 2021:2287:23-39.
doi: 10.1007/978-1-0716-1315-3_2.

Applications of Doubled Haploids in Plant Breeding and Applied Research

Jens Weyen  1
Affiliations
Review

Applications of Doubled Haploids in Plant Breeding and Applied Research

Jens Weyen. Methods Mol Biol. 2021.

Abstract

Manifold and diverse applications of doubled haploid (DH) plants have emerged in academy and in the plant breeding industry since the first discovery of a haploid mutant in the Jimson Weed (Datura stramonium), followed by the first reports about anther culture in the same species, maternal haploids by wide crosses in tobacco (Nicotiana tabacum L.) and barley (Hordeum vulgare L.), interspecific hybridization, ovary culture (gynogenesis), isolated microspore culture, and more recently the CENH3 approach in thale cress (Arabidopsis thaliana L.) and other species. Research and development efforts were and are still significant in both user groups. Luckily, often academic and industrial partners cooperate in challenging and sometimes voluminous projects worldwide. Not only to develop innovative DH protocols and technologies per se, but also to exploit the advantages of DH plants in a huge variety of research and development experiments. This review concentrates not on the DH technologies per se, but on the application of DHs in plant-related research and development projects.

Keywords: Biostatistics; Breeding strategy; DH; Doubled haploids; Genetic variability; Genome editing; Genomic selection; Homozygosity; Molecular markers; Recombination; Selection.

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References

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