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Review
. 2021:2289:3-22.
doi: 10.1007/978-1-0716-1331-3_1.

Centromere Engineering as an Emerging Tool for Haploid Plant Production: Advances and Challenges

Raheleh Karimi-Ashtiyani  1
Affiliations
Review

Centromere Engineering as an Emerging Tool for Haploid Plant Production: Advances and Challenges

Raheleh Karimi-Ashtiyani. Methods Mol Biol. 2021.

Abstract

Haploid production is of great importance in plant breeding programs. Doubled haploid technology accelerates the generation of inbred lines with homozygosity in all loci in a single year. Haploids can be induced in vitro via cultivating the haploid gametes or in vivo through inter- and intraspecific hybridization. Haploid induction through centromere engineering is a novel system that is theoretically applicable to many plant species. The present review chapter discusses the proposed molecular mechanisms of selective chromosome elimination in early embryogenesis and the effects of kinetochore component modifications on proper chromosome segregation. Finally, the advantages and limitations of the CENH3-mediated haploidization approach and its applications are highlighted.

Keywords: CENH3; CRISPR/Cas9; Centromere; Haploid inducer line; Kinetochore.

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References

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