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. 2021 Jul 16;105(4):1093-1096.
doi: 10.4269/ajtmh.21-0303.

Molecular Detection of Mutations in the Propeller Domain of Kelch 13 and pfmdr1 Copy Number Variation in Plasmodium falciparum Isolates from Thailand Collected from 2002 to 2007

Chaiyaporn Chaisatit  1 , Piyaporn Sai-Ngam  1 , Sasikanya Thaloengsok  1 , Sabaithip Sriwichai  1 , Krisada Jongsakul  1 , Mark Fukuda  2 , Michele Spring  1 , Mariusz Wojnarski  1 , Norman Waters  1 , Paphavee Lertsethtakarn  1 , Brian A Vesely  1
Affiliations

Molecular Detection of Mutations in the Propeller Domain of Kelch 13 and pfmdr1 Copy Number Variation in Plasmodium falciparum Isolates from Thailand Collected from 2002 to 2007

Chaiyaporn Chaisatit et al. Am J Trop Med Hyg. .

Abstract

We determined the prevalence of Kelch 13 mutations and pfmdr1 copy number in samples collected from the Thailand-Myanmar border, the Thailand-Cambodia border, and southern Thailand from 2002 to 2007. C580Y was the most prevalent in Trat (Thailand-Cambodia border) and Ranong (Thailand-Myanmar border) at 42% (24/57) and 13% (6/48), respectively. Less predominant mutations were also identified including R539T (7%, 4/57) and Y493H (2%, 1/57) in Trat, P574L (6%, 3/48) and P553L (2%, 1/48) in Ranong, and N537I and D452E (7%, 1/15) in Sangkhlaburi (Thailand-Myanmar border). Samples from Mae sot (33%, 11/33) harbored the highest percentage of multiple pfmdr1 copies, followed by Trat (18%, 10/57), Chiang Dao in 2003 (13%, 4/30), Phang Nga (5%, 2/44), and Chiang Dao in 2002 (4%, 1/26). This retrospective study provides geographic diversity of K13 and pfmdr1 copies and the emergence of these molecular markers in Thailand, an important background information for future surveillance in the region.

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Figures

Figure 1.
Figure 1.
Geographic distribution of Plasmodium falciparum K13 mutations collected in six provinces in Thailand from 2002 to 2007. The results are depicted on the spatial distribution of P. falciparum cases in Thailand in 2007 (Malaria Atlas Project). This figure appears in color at www.ajtmh.org.
Figure 2.
Figure 2.
Pfmdr1 copy number and K13 mutations in Plasmodium falciparum samples in six provinces in Thailand from 2002 to 2007. This figure appears in color at www.ajtmh.org.
See this image and copyright information in PMC

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