Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2021 Jul 20;118(29):e2110553118.
doi: 10.1073/pnas.2110553118.

Dynamics ante portas

Jochem H Smit  1 , Guillaume Roussel  1 , Anastassios Economou  2
Affiliations

Dynamics ante portas

Jochem H Smit et al. Proc Natl Acad Sci U S A. .
No abstract available

PubMed Disclaimer

Conflict of interest statement

The authors declare no competing interest.

Figures

Fig. 1.
Fig. 1.
Single-molecule pipeline revealing gate dynamics in SecY (see text for details). (Top Left) The heterotrimeric SecYEG translocon is shown as a cylinder embedded in the lipid bilayer. A narrow equatorial constriction regulates vectorial protein transport and transmembrane helices TM2 and 7 form the “lateral gate” that faces the bilayer. The translocon was extracted from its phospholipid environment into nanodiscs (I) and fluorescent probes that can act as a donor (D)–acceptor (A) pair in FRET were placed stochastically on TM2 and elsewhere on the protein. Relative distances between the probes were measured by TIRF and PIE and revealed at least four different states (II; darker hues indicate more populated states) that were modulated by (III, Top) the addition of a translocating nascent chain-ribosome complex (RNC); (III, Middle) single point mutations (colored stars), gain-of-function (Prl: I90N and S282R) or loss-of-function (P84L or P276S or P287L); and (III, Bottom) the interacting chaperone/insertase YidC.
See this image and copyright information in PMC

Comment on

  • doi: 10.1073/pnas.2100474118

References

    1. Mercier E., Wang X., Maiti M., Wintermeyer W., Rodnina M. V., Lateral gate dynamics of the bacterial translocon during cotranslational membrane protein insertion. Proc. Natl. Acad. Sci. U.S.A. 118, e2100474118 (2021). - PMC - PubMed
    1. Dalal K., Duong F., The SecY complex: Conducting the orchestra of protein translocation. Trends Cell Biol. 21, 506–514 (2011). - PubMed
    1. Van den Berg B., et al. ., X-ray structure of a protein-conducting channel. Nature 427, 36–44 (2004). - PubMed
    1. du Plessis D. J. F., Berrelkamp G., Nouwen N., Driessen A. J. M., The lateral gate of SecYEG opens during protein translocation. J. Biol. Chem. 284, 15805–15814 (2009). - PMC - PubMed
    1. Bondar A.-N., del Val C., Freites J. A., Tobias D. J., White S. H., Dynamics of SecY translocons with translocation-defective mutations. Structure 18, 847–857 (2010). - PMC - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources