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Review
. 2021 Jul;5(7):643-656.
doi: 10.1038/s41551-021-00760-7. Epub 2021 Jul 16.

CRISPR-based diagnostics

Michael M Kaminski  1   2 , Omar O Abudayyeh  3   4 , Jonathan S Gootenberg  3   4 , Feng Zhang  3   4   5   6   7   8 , James J Collins  9   10   11   12
Affiliations
Review

CRISPR-based diagnostics

Michael M Kaminski et al. Nat Biomed Eng. 2021 Jul.

Abstract

The accurate and timely diagnosis of disease is a prerequisite for efficient therapeutic intervention and epidemiological surveillance. Diagnostics based on the detection of nucleic acids are among the most sensitive and specific, yet most such assays require costly equipment and trained personnel. Recent developments in diagnostic technologies, in particular those leveraging clustered regularly interspaced short palindromic repeats (CRISPR), aim to enable accurate testing at home, at the point of care and in the field. In this Review, we provide a rundown of the rapidly expanding toolbox for CRISPR-based diagnostics, in particular the various assays, preamplification strategies and readouts, and highlight their main applications in the sensing of a wide range of molecular targets relevant to human health.

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