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Published Erratum
. 2021 Jul:69:103469.
doi: 10.1016/j.ebiom.2021.103469. Epub 2021 Jul 14.

Corrigendum to "A miR-567-PIK3AP1-PI3K/AKT-c-Myc feedback loop regulates tumour growth and chemoresistance in gastric cancer" [EBioMedicine 44 (2019) 311- 321]

Feifei Zhang  1 , Kaitao Li  1 , Xueqing Yao  2 , Hui Wang  3 , Weidong Li  3 , Juan Wu  3 , Mingyi Li  4 , Rui Zhou  5 , Lijun Xu  5 , Liang Zhao  6
Affiliations
Published Erratum

Corrigendum to "A miR-567-PIK3AP1-PI3K/AKT-c-Myc feedback loop regulates tumour growth and chemoresistance in gastric cancer" [EBioMedicine 44 (2019) 311- 321]

Feifei Zhang et al. EBioMedicine. 2021 Jul.
No abstract available

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Figures

Fig 2
Fig. 2
miR-567 suppresses GC cell proliferation and increases drug sensitivity in vitro and in vivo. FACS assays (A) and EdU incorporation assays (B) of GC cells were performed after transfected with miR-567 mimic or anti-miR-567, Student's t-test, mean ± SD, *P<0.05; **P<0.01; *** P<0.001. (C&D) Dose-response curves of MGC803 and BGC823 treated with 5-FU for 36 h or oxaliplatin for 24 h, the cells were previously transfected with miR-NC, miR-567, anti-miR-NC or anti-miR-567. Parametric generalized linear model with random effects, Student's t-test, mean ± SD, *P<0.05; **P<0.01; *** P<0.001. (E) Western blot experiments were used to analyse the expression of c-casp3, casp3, c-PARP, PARP, c-casp9, casp9, p21, CDK4, CDK6 and CyclinD1 after miR-567 knockdown and overexpression in MGC803 and BGC823 cells. (F)In vivo image detection of the xenograft tumour growth. Growth curve was measured and drawn, *P<0.05. The tumour sections were under IHC staining using antibodies against Ki-67. Quantification of Ki67 staining of the xenograft tumours (right). ***P<0.001.
Fig 3
Fig. 3
PIK3AP1 is the direct target of miR-567 and promotes GC cell proliferation and increases drug sensitivity. (A) Real-time PCR analysis were performed to detect the mRNA expression of candidate genes in MGC803 and BGC823 cells transfected with miR-567 mimic, Student's t-test, mean ± SD. (B) miR-567 and its putative binding sequences in the 3′UTR of PIK3AP1. A mutation was generated in the complementary site that bound to the seed region of miR-567. Luciferase reporter assay was used to determine miR-567 direct targeting the PIK3AP1 3′UTR, Student's t-test, mean ± SD, ***P<0.001. (B) Western blot analysis were performed to detect the protein expression of PIK3AP1 in MGC803 and BGC823 cells, both transfected with miR-567 mimic or anti-miR-567. CCK-8 assay (C), colony formation assay (D), FACS assays (E) and EdU incorporation assays (F) of GC cells were performed after transfected with PIK3AP1 or pENTER vector, Student's t-test, mean ± SD, *P < 0.05; **P < 0.01. (H) Western blot experiments were used to analyse the expression of pro-apoptosis proteins, cell cycle maker and related proteins in PI3K/AKT pathway after miR-567 knockdown and overexpression in MGC803 and BGC823 cells. (I) Dose-response curves of MGC803 and BGC823 treated with 5-FU for 36 h or oxaliplatin for 24 h, the cells were previously transfected with PIK3AP1 and pENTER. Parametric generalized linear model with random effects, Student's t-test, mean ± SD, *P<0.05; **P<0.01; *** P<0.001.
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