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. 2021 Jul 5;26(13):4101.
doi: 10.3390/molecules26134101.

Assessment of the In Vivo Release and Biocompatibility of Novel Vesicles Containing Zinc in Rats

Liliana Mititelu-Tartau  1 , Maria Bogdan  2 , Daniela Angelica Pricop  3 , Beatrice Rozalina Buca  1 , Ana-Maria Pauna  1 , Lorena Anda Dijmarescu  4 , Ana-Maria Pelin  5 , Liliana Lacramioara Pavel  6 , Gratiela Eliza Popa  7
Affiliations

Assessment of the In Vivo Release and Biocompatibility of Novel Vesicles Containing Zinc in Rats

Liliana Mititelu-Tartau et al. Molecules. .

Abstract

This paper is focused on the in vivo release and biocompatibility evaluation in rats of some novel systems entrapping zinc chloride in lipid vesicles. The particles were prepared by zinc chloride immobilization inside lipid vesicles made using phosphatidylcholine, stabilized with 0.5% chitosan solution, and dialyzed for 10 h to achieve a neutral pH. The submicrometric systems were physico-chemically characterized. White Wistar rats, assigned into four groups of six animals each, were treated orally with a single dose, as follows: Group I (control): deionized water 0.3 mL/100 g body weight; Group II (Zn): 2 mg/kg body weight (kbw) zinc chloride; Group III (LV-Zn): 2 mg/kbw zinc chloride in vesicles; Group IV (LVC-Zn): 2 mg/kbw zinc chloride in vesicles stabilized with chitosan. Haematological, biochemical, and immune parameters were assessed after 24 h and 7 days, and then liver fragments were collected for histopathological examination. The use of zinc submicrometric particles-especially those stabilized with chitosan-showed a delayed zinc release in rats. No substantial changes to blood parameters, plasma biochemical tests, serum complement activity, or peripheral neutrophils phagocytic capacity were noted; moreover, the tested substances did not induce liver architectural disturbances. The obtained systems provided a delayed release of zinc, and showed good biocompatibility in rats.

Keywords: biocompatibility; lipid vesicles; rats; zinc chloride.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Size distribution frequency of Zn vesicles in solution.
Figure 2
Figure 2
Distribution of the Zn vesicles’zeta potential.
Figure 3
Figure 3
DIC (20×) optical microscopy images representing (a) LV-Zn and (b) LVC-Zn before dialysis.
Figure 4
Figure 4
The in vitro release of zinc (molar concentration) from LVC-Zn. Values are expressed as mean ± S.D.
Figure 5
Figure 5
UV–VIS Spectra for Zn (a) and LVC-Zn (b) in solution.
Figure 6
Figure 6
Variation of serum Zn levels after the administration of LV-Zn and LVC-Zn in rats; * p < 0.01 vs. baseline; ♦ p < 0.01 vs. Zn.
Figure 7
Figure 7
Histological liver architecture in animals treated with deionized water (a), Zn (b), LV-Zn (c), and LVC-Zn (d); H&E stain ×10.
See this image and copyright information in PMC

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