Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2021 Dec;12(1):3993-4003.
doi: 10.1080/21655979.2021.1950278.

Long noncoding RNA SNHG20 regulates cell migration, invasion, and proliferation via the microRNA-19b-3p/RAB14 axis in oral squamous cell carcinoma

Xiaomi Zhu  1 , Hanzhong Zhang  2 , Juan Xu  3
Affiliations

Long noncoding RNA SNHG20 regulates cell migration, invasion, and proliferation via the microRNA-19b-3p/RAB14 axis in oral squamous cell carcinoma

Xiaomi Zhu et al. Bioengineered. 2021 Dec.

Abstract

Oral squamous cell carcinoma (OSCC) is one of the most common digestive tumors, which has high mortality rate. Long non-coding RNAs (lncRNA) and MicroRNAs (miRNAs) are associated with the cell cycle and differentiation during the occurrence and development of malignant tumors. This research aimed to investigate the effects of lncRNA SNHG20 on the progress of oral squamous cell carcinoma (OSCC) cells. Ninety pairs of tumor tissues and paracancerous tissues were collected from patients with OSCC and the CAL27 and SCC25 OSCC cells were selected for the following experiments. RT-qPCR was used for detecting the expression of SNHG20, miR-19b-3p, and RAB14. Western blotting was used to detect the protein levels of RAB14. MTT assay was employed to assess cell proliferation. Transwell assay was used to determine the cell migration and invasion abilities. Furthermore, luciferase reporter and RNA pull-down assays were used to verify the binding of SNHG20/RAB14 to miR-19b-3p. Then, the function of the SNHG20/miR-19b-3p/RAB14 axis in OSCC was explored. The results indicated that lncRNA SNHG20 was upregulated in the tissues. Furthermore, bioinformatic analysis showed that both SNHG20 and RAB14 could bind to miR-19b-3p. RAB14 was upregulated, and miR-19b-3p was downregulated in the tissues. The knockdown of SNHG20 inhibited cell proliferation, migration, and invasion. Contrarily, the knockdown of miR-19b-3p reversed the effects of si-SNHG20 on cell proliferation, migration, and invasion, and the overexpression of RAB14 reversed the effects of miR-19b-3p mimic on the cell biological functions. LncRNA SNHG20 affects cell proliferation, migration, and invasion via the miR-19b-3p/RAB14 axis in OSCC.

Keywords: Lncrna snhg20; OSCC; RAB14; miR-19b-3p; proliferation.

PubMed Disclaimer

Conflict of interest statement

No potential conflict of interest was reported by the author(s).

Figures

None
Graphical abstract
Figure 1.
Figure 1.
LncRNA SNHG20 was overexpressed in OSCC tissues and CAL27 and SCC25 cells
Figure 2.
Figure 2.
The knockdown of lncRNA SNHG20 decreased the proliferation, migration, and invasion of SCC25 and CAL27 cells
Figure 3.
Figure 3.
LncRNA SNHG20 regulates the OSCC cell functions by sponging miR-19b-3p
Figure 4.
Figure 4.
The knockdown of miR-19b-3p reversed the effect of si-SNHG20 on the proliferation, migration, and invasion of SCC25 and CAL27 cells
Figure 5.
Figure 5.
RAB14 is the target gene of miR-19b-3p in OSCC
Figure 6.
Figure 6.
Overexpression of RAB14 reversed the effect of miR-19b-3p mimic on the proliferation, migration, and invasion of SCC25 and CAL27 cells
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Morgensztern D, Campo MJ, Dahlberg SE, et al. Molecularly targeted therapies in non–small-cell lung cancer annual update 2014. J Thorac Oncol. 2015;10:S1–S63. - PMC - PubMed
    1. Alberg AJ, Brock MV, Ford JG, et al. Epidemiology of lung cancer: diagnosis and management of lung cancer, 3rd ed: American College of chest physicians evidence-based clinical practice guidelines. Chest. 2013;143:e1S–e29S. - PMC - PubMed
    1. Shtivelman E, Hensing T, Simon GR, et al. Molecular pathways and therapeutic targets in lung cancer. Oncotarget. 2014;5(6):1392–1433. - PMC - PubMed
    1. Wu X, Wu G, Yao X, et al. The clinicopathological significance and ethnic difference of FHIT hypermethylation in non-small-cell lung carcinoma: a meta-analysis and literature review. Drug Des Devel Ther. 2016;10:699–709. - PMC - PubMed
    1. Lemjabbar-Alaoui H, Hassan OU, Yang Y, et al. Lung cancer: biology and treatment options, Biochimica et biophysica acta. Rev Cancer. 2015;1856:189–210. - PMC - PubMed

Publication types

MeSH terms