Zn-based physiometacomposite nanoparticles: distribution, tolerance, imaging, and antiviral and anticancer activity

Robert K DeLong^{1

2}, Ryan Swanson^{1

2}, Megan C Niederwerder³, Pratiksha Khanal³, Santosh Aryal^{4

5}, Ramesh Marasini^{2

4}, Majid Jaberi-Douraki⁶, Heman Shakeri⁶, Reza Mazloom⁶, Sarah Schneider^{3

7}, Steve Ensley^{1

3}, Lane L Clarke^{8

9}, Rowena A Woode⁹, Sarah Young¹⁰, Sagar Rayamajhi⁴, Tracy Miesner¹¹, Mary L Higginbotham¹², Zhoumeng Lin^{1

13}, Tej Shrestha^{1

2}, Kartik Ghosh¹⁴, Garry Glaspell¹⁵, Elza N Mathew^{1

16}

Affiliations

¹ Department of Anatomy & Physiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506, USA.
² Nanotechnology Innovation Center, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506, USA.
³ Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506, USA.
⁴ Department of Chemistry, Kansas State University, Manhattan, KS 66506, USA.
⁵ Department of Pharmaceutical Sciences and Health Outcomes, The Ben and Maytee Fisch College of Pharmacy, University of Texas at Tyler, Tyler, TX 75799, USA.
⁶ 1DATA Consortium, & Department of Mathematics, Kansas State University Olathe, Olathe, KS 66061, USA.
⁷ Department of Pathology, College of Veterinary Medicine, University of Georgia, Athens, GA 30602, USA.
⁸ Dalton Cardiovascular Research Center, University of Missouri, Columbia, MO 65211, USA.
⁹ Department of Biomedical Sciences, University of Missouri, Columbia, MO 65211, USA.
¹⁰ Department of Veterinary Pathobiology, University of Missouri, Columbia, MO 65211, USA.
¹¹ Comparative Medicine Group, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506, USA.
¹² Department of Clinical Sciences, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506, USA.
¹³ Institute for Computational Comparative Medicine, Kansas State University Manhattan, KS 66061, USA.
¹⁴ Department of Physics, Astronomy & Materials Science, Missouri State University, Springfield, MO 65897, USA.
¹⁵ US Army Corps of Engineers Engineer Research & Development Center, Alexandria, VA 22315, USA.
¹⁶ University of Massachusetts Medical School, Worcester, MA 01605, USA.

PMID: 34282923
DOI: 10.2217/nnm-2021-0179

Zn-based physiometacomposite nanoparticles: distribution, tolerance, imaging, and antiviral and anticancer activity

Robert K DeLong et al. Nanomedicine (Lond). 2021 Sep.

. 2021 Sep;16(21):1857-1872.

doi: 10.2217/nnm-2021-0179. Epub 2021 Jul 20.

Authors

Affiliations

¹ Department of Anatomy & Physiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506, USA.
² Nanotechnology Innovation Center, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506, USA.
³ Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506, USA.
⁴ Department of Chemistry, Kansas State University, Manhattan, KS 66506, USA.
⁵ Department of Pharmaceutical Sciences and Health Outcomes, The Ben and Maytee Fisch College of Pharmacy, University of Texas at Tyler, Tyler, TX 75799, USA.
⁶ 1DATA Consortium, & Department of Mathematics, Kansas State University Olathe, Olathe, KS 66061, USA.
⁷ Department of Pathology, College of Veterinary Medicine, University of Georgia, Athens, GA 30602, USA.
⁸ Dalton Cardiovascular Research Center, University of Missouri, Columbia, MO 65211, USA.
⁹ Department of Biomedical Sciences, University of Missouri, Columbia, MO 65211, USA.
¹⁰ Department of Veterinary Pathobiology, University of Missouri, Columbia, MO 65211, USA.
¹¹ Comparative Medicine Group, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506, USA.
¹² Department of Clinical Sciences, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506, USA.
¹³ Institute for Computational Comparative Medicine, Kansas State University Manhattan, KS 66061, USA.
¹⁴ Department of Physics, Astronomy & Materials Science, Missouri State University, Springfield, MO 65897, USA.
¹⁵ US Army Corps of Engineers Engineer Research & Development Center, Alexandria, VA 22315, USA.
¹⁶ University of Massachusetts Medical School, Worcester, MA 01605, USA.

PMID: 34282923
DOI: 10.2217/nnm-2021-0179

Abstract

The aim of this study was to investigate the distribution, tolerance, and anticancer and antiviral activity of Zn-based physiometacomposites (PMCs). Manganese, iron, nickel and cobalt-doped ZnO, ZnS or ZnSe were synthesized. Cell uptake, distribution into 3D culture and mice, and biochemical and chemotherapeutic activity were studied by fluorescence/bioluminescence, confocal microscopy, flow cytometry, viability, antitumor and virus titer assays. Luminescence and inductively coupled plasma mass spectrometry analysis showed that nanoparticle distribution was liver >spleen >kidney >lung >brain, without tissue or blood pathology. Photophysical characterization as ex vivo tissue probes and LL37 peptide, antisense oligomer or aptamer delivery targeting RAS/Ras binding domain (RBD) was investigated. Treatment at 25 μg/ml for 48 h showed ≥98-99% cell viability, 3D organoid uptake, 3-log inhibition of β-Galactosidase and porcine reproductive respiratory virus infection. Data support the preclinical development of PMCs for imaging and delivery targeting cancer and infectious disease.

Keywords: anticancer activity; antisense oligomer delivery; antiviral activity; aptamer delivery; nanomedicine; peptide delivery.

PubMed Disclaimer

Publication types

Actions
Actions

MeSH terms

Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions

Substances

Actions
Actions

LinkOut - more resources

Full Text Sources
- Atypon

Save citation to file

Email citation

Add to Collections

Add to My Bibliography

Your saved search

Create a file for external citation management software

Your RSS Feed

Zn-based physiometacomposite nanoparticles: distribution, tolerance, imaging, and antiviral and anticancer activity

Affiliations

Zn-based physiometacomposite nanoparticles: distribution, tolerance, imaging, and antiviral and anticancer activity

Authors

Affiliations

Abstract

Publication types

MeSH terms

Substances

LinkOut - more resources

Full Text Sources