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. 2018 May 20;8(10):e2844.
doi: 10.21769/BioProtoc.2844.

Quantification of Salicylic Acid (SA) and SA-glucosides in Arabidopsis thaliana

Valérie Allasia  1 , Benoit Industri  1 , Michel Ponchet  1 , Michaël Quentin  1 , Bruno Favery  1 , Harald Keller  1
Affiliations

Quantification of Salicylic Acid (SA) and SA-glucosides in Arabidopsis thaliana

Valérie Allasia et al. Bio Protoc. .

Abstract

Homeostasis between the cytoplasmic plant hormone salicylic acid (SA) and its' inactive, vacuolar storage forms, SA-2-O-β-D-glucoside (SAG) and SA-β-D-Glucose Ester (SGE), regulates the fine-tuning of defense responses to biotrophic pathogens in Arabidopsis thaliana. This protocol describes a simplified, optimized procedure to extract and quantify free SA and total hydrolyzable SA in plant tissues using a classical HPLC-based method.

Keywords: Arabidopsis thaliana; Defense hormone; HPLC; SA-glucoside; Salicylic acid.

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Figures

Figure 1.
Figure 1.. Flowchart for the preparation of SA-containing samples
Figure 2.
Figure 2.. UV spectra for SA (A) and OAA (B), and chromatograms of fluorescence-detected free SA (C) and SA-hydrolysates (D) in extracts from Arabidopsis seedlings, four days after inoculation with the Hyaloperonospora arabidopsidis isolate Waco 9
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