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. 2021 Jul 19;36(6):e360605.
doi: 10.1590/ACB360605. eCollection 2021.

Comparison of two different biomaterials in the bone regeneration (15, 30 and 60 days) of critical defects in rats

Patricia Brassolatti  1 , Paulo Sérgio Bossini  2 , Ana Laura Martins de Andrade  3 , Genoveva Lourdes Flores Luna  4 , Juliana Virginio da Silva  5 , Luciana Almeida-Lopes  6 , Marcos Aurélio Napolitano  7 , Lucimar Retto da Silva de Avó  8 , Ângela Merice de Oliveira Leal  8 , Fernanda de Freitas Anibal  9
Affiliations

Comparison of two different biomaterials in the bone regeneration (15, 30 and 60 days) of critical defects in rats

Patricia Brassolatti et al. Acta Cir Bras. .

Abstract

Purpose: To evaluate and compare two types of different scaffolds in critical bone defects in rats.

Methods: Seventy male Wistar rats (280 ± 20 grams) divided into three groups: control group (CG), untreated animals; biomaterial group 1 (BG1), animals that received the scaffold implanted hydroxyapatite (HA)/poly(lactic-co-glycolic) acid (PLGA); and biomaterial group 2 (BG2), animals that received the scaffolds HA/PLGA/Bleed. The critical bone defect was induced in the medial region of the skull calotte with the aid of an 8-mm-diameter trephine drill. The biomaterial was implanted in the form of 1.5 mm thick scaffolds, and samples were collected after 15, 30 and 60 days. Non-parametric Mann-Whitney test was used, with the significance level of 5% (p ≤ 0.05).

Results: Histology revealed morphological and structural differences of the neoformed tissue between the experimental groups. Collagen-1 (Col-1) findings are consistent with the histological ones, in which BG2 presented the highest amount of fibers in its tissue matrix in all evaluated periods. In contrast, the results of receptor activator of nuclear factor kappa-Β ligand (Rank-L) immunoexpression were higher in BG2 in the periods of 30 and 60 days, indicating an increase of the degradation of the biomaterial and the remodeling activity of the bone.

Conclusions: The properties of the HA/PLGA/Bleed scaffold were superior when compared to the scaffold composed only by HA/PLGA.

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Conflict of interest statement

Conflict of interest: Nothing to declare.

Figures

Figure 1
Figure 1. Scaffolds of the biomaterials used in this study. (a) HA/PLGA scaffold; (b) HA/PLGA/Bleed scaffold. The scaffolds of both experimental groups had 8 mm of diameter and were 1.5 mm thick.
Figure 2
Figure 2. Photomicrographs representative of experimental groups. (a) Control group 15 days, (b) Biomaterial group 1 at 15 days, (c) Biomaterial group 2 at 15 days, (d) Control group at 30 days, (e) Biomaterial group 1 at 30 days,(f) Biomaterial group 2 at 30 days, (g) Control group at 60 days, (h) Biomaterial group 1 at 60 days, (i) biomaterial group 2 at 60 days. Coloration: hematoxylin and eosin (HE), bar = 40 µm, objective increase x10.
Figure 3
Figure 3. Representative graph of morphometric analysis **.
Figure 4
Figure 4. Representative graph of imunohistochemistry analysis of Collagen-I *,**.
Figure 5
Figure 5. Representative graph of imunohistochemistry analysis of Rank-L*,
See this image and copyright information in PMC

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