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. 2021:2320:171-180.
doi: 10.1007/978-1-0716-1484-6_17.

A Method for Contraction Force Measurement of hiPSC-Derived Engineered Cardiac Tissues

Yuya Fujiwara  1   2 , Kohei Deguchi  2   3 , Kenji Miki  1 , Tomoyuki Nishimoto  2   3 , Yoshinori Yoshida  4
Affiliations

A Method for Contraction Force Measurement of hiPSC-Derived Engineered Cardiac Tissues

Yuya Fujiwara et al. Methods Mol Biol. 2021.

Abstract

Engineered cardiac tissue (ECT) derived from human induced pluripotent stem cells (iPSCs) can replicate human heart in vitro and be applied to drug discovery and heart disease models. The contraction force of ECT is an important indicator of its function and of the disease phenotype. Here we describe a construction method of ECT using the Flexcell® Tissue Train® culture system and a contraction force measurement method based on the Frank-Starling law.

Keywords: Cardiomyocytes; Contraction force; Engineered cardiac tissue; Frank–Starling law; Human induced pluripotent stem cells; Twitch force.

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