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. 2021 Jul 8:12:653927.
doi: 10.3389/fgene.2021.653927. eCollection 2021.

Whole-Genome Resequencing to Study Brucellosis Susceptibility in Sheep

Xiaolong Li  1 , Qingmin Wu  2 , Xiaoxue Zhang  1   3 , Chong Li  1   3 , Deyin Zhang  1 , Guoze Li  1 , Yukun Zhang  1 , Yuan Zhao  1 , Zhaoguo Shi  1 , Weimin Wang  1 , Fadi Li  1   3   4
Affiliations

Whole-Genome Resequencing to Study Brucellosis Susceptibility in Sheep

Xiaolong Li et al. Front Genet. .

Abstract

Brucellosis is a zoonotic disease and a major public health problem. However, the genetic mechanism of brucellosis in sheep remains unclear. In this study, serum samples were collected from 6,358 sheep from the F2 population (Dorper sheep ♂ × Hu sheep ♀), and antibody levels were continuously measured at 14 days and 1, 2, 3, 4, 5, 6, 7, 8, 9, and 10 months after administration of brucellosis vaccine. Finally, 19 brucellosis-resistant group (BRG) sheep and 22 brucellosis-susceptible group sheep (BSG) were screened for whole-genome sequencing. Using the fixation index, Fisher's exact test, and chi-square test, a total of 205 candidate SNP sites were identified. Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis suggested that 138 candidate genes were significantly enriched in adherens junction (CTNNA3, PARD3, and PTPRM), cell adhesion molecules (NLGN1, CNTNAP2, NCAM1, and PTPRM), salivary secretion (LOC101102109, PRKG1, and ADCY2), and hippo signaling pathway (CTNNA3, YAP1, and PARD3). These findings provide valuable molecular markers for brucellosis resistance breeding in sheep and novel insights into the genetic mechanism of brucellosis resistance.

Keywords: FST; Fisher’s exact test; brucellosis; chi-square test; sheep; whole-genome resequencing.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

FIGURE 1
FIGURE 1
Brucella antibody levels in sheep from the brucellosis-resistant group (BRG) and brucellosis-susceptible group (BSG). Blue and red represent the antibody levels of BRG and BSG, respectively, ∗∗highly significant difference (Mann–Whitney U test, p < 0.01).
FIGURE 2
FIGURE 2
Genome-wide distribution of FST (A), Fisher’s exact test (B), and chi-square test (C). The horizontal black line in the figure shows the values for the first 1,000.
FIGURE 3
FIGURE 3
Shared SNP sites Venn diagram of three statistical methods.
FIGURE 4
FIGURE 4
The top 20 KEGG enrichment pathways.
See this image and copyright information in PMC

References

    1. Ackermann M. A., Petrosino J. M., Manring H. R., Wright P., Shettigar V., Kilic A., et al. (2017). TGF-β1 affects cell-cell adhesion in the heart in an NCAM1-dependent mechanism. J. Mol. Cell. Cardiol. 112 49–57. 10.1016/j.yjmcc.2017.08.015 - DOI - PMC - PubMed
    1. Al Jindan R. (2021). Scenario of pathogenesis and socioeconomic burden of human brucellosis in Saudi Arabia. Saudi J. Biol. Sci. 28 272–279. 10.1016/j.sjbs.2020.09.059 - DOI - PMC - PubMed
    1. Amjadi O., Rafiei A., Mardani M., Zafari P., Zarifian A. (2019). A review of the immunopathogenesis of brucellosis. Infect. Dis. 51 321–333. 10.1080/23744235.2019.1568545 - DOI - PubMed
    1. Barazeghi E., Hellman P., Westin G., Stålberg P. (2019). PTPRM, a candidate tumor suppressor gene in small intestinal neuroendocrine tumors. Endocr. Connect. 8 1126–1135. 10.1530/EC-19-0279 - DOI - PMC - PubMed
    1. Batikhan H., Gokcan M. K., Beder E., Akar N., Ozturk A., Gerceker M. (2010). Association of the tumor necrosis factor-alpha -308 G/A polymorphism with nasal polyposis. Eur. Arch. Otorhinolaryngol. 267 903–908. 10.1007/s00405-009-1167-5 - DOI - PubMed