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. 2017:4:e1349.
Epub 2017 Jun 21.

Triggerable RNA nanodevices

Justin Halman  1 , Emily Satterwhite  1 , Jaclyn Smollett  1 , Eckart Bindewald  2 , Lorena Parlea  3 , Mathias Viard  3   2 , Paul Zakrevsky  3 , Wojciech K Kasprzak  2 , Kirill A Afonin  1 , Bruce A Shapiro  3
Affiliations

Triggerable RNA nanodevices

Justin Halman et al. RNA Dis. 2017.

Abstract

The targeted and conditional activation of pharmaceuticals is an increasingly important feature in modern personalized medicine. Nucleic acid nanoparticles show tremendous potential in this exploit due to their programmability and biocompatibility. Among the most powerful nucleic acid specific treatments is RNA interference-based therapeutics. RNA interference is a naturally occurring phenomenon in which specific genes are effectively silenced. Recently we have developed two different strategies based on customized multivalent nucleic acid nanoparticles with the ability to conditionally activate RNA interference in diseased cells as well as elicit detectable fluorescent responses.[1,2] These novel technologies can be further utilized for the simultaneous delivery and conditional intracellular activation of multiple therapeutic and biosensing functions to combat various diseases.

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Figures

Figure 1.
Figure 1.. Schematic representation of two alternative ways, described in highlighted papers, to conditionally activate various functionalities intracellularly.
A: corresponds to the hybrid approach and B: corresponds to the two stranded switch approach.
See this image and copyright information in PMC

References

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    1. Afonin KA, Viard M, Tedbury P, Bindewald E, Parlea L, Howington M, et al. The Use of Minimal RNA Toeholds to Trigger the Activation of Multiple Functionalities. Nano letters 2016; 16:1746–1753. - PMC - PubMed
    1. Afonin KA, Grabow WW, Walker FM, Bindewald E, Dobrovolskaia MA, Shapiro BA, et al. Design and self-assembly of siRNA-functionalized RNA nanoparticles for use in automated nanomedicine. Nat Protoc 2011; 6:2022–2034. - PMC - PubMed
    1. Afonin KA, Kasprzak WK, Bindewald E, Kireeva M, Viard M, Kashlev M, et al. In silico design and enzymatic synthesis of functional RNA nanoparticles. Acc Chem Res 2014; 47:1731–1741. - PMC - PubMed
    1. Afonin KA, Viard M, Koyfman AY, Martins AN, Kasprzak WK, Panigaj M, et al. Multifunctional RNA nanoparticles. Nano letters 2014; 14:5662–5671. - PMC - PubMed

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