doi: 10.1073/pnas.75.1.294.
Mutant RNA polymerase of Escherichia coli terminates transcription in strains making defective rho factor
- PMID: 343107
- PMCID: PMC411233
- DOI: 10.1073/pnas.75.1.294
Item in Clipboard
Mutant RNA polymerase of Escherichia coli terminates transcription in strains making defective rho factor
Proc Natl Acad Sci U S A.
1978 Jan.
Abstract
We have isolated a rifampicin-resistant mutant of Escherichia coli RNA polymerase that restores transcription termination in strains with a defective rho protein. In such strains, the mutant RNA polymerase terminates transcription at normally rho-dependent sites at the end of the trp operon, in bacteriophage lambda, and within the lac operon. In addition, a strain with this mutant RNA polymerase remains viable with an amber mutation in rho, whereas a strain with wild-type RNA polymerase does not. These results suggest that the mutant RNA polymerase can terminate transcription at normally rho-dependent sites in the absence of rho.
Similar articles
-
The attenuator of the tryptophan operon in E.coli: rho-mediated release of RNA polymerase from a transcription termination complex in vitro.Nucleic Acids Res. 1978 Dec;5(12):4613-23. Nucleic Acids Res. 1978. PMID: 370776 Free PMC article.
-
Interaction of RNA polymerase and rho in transcription termination: coupled ATPase.Proc Natl Acad Sci U S A. 1978 Oct;75(10):4828-32. doi: 10.1073/pnas.75.10.4828. Proc Natl Acad Sci U S A. 1978. PMID: 154103 Free PMC article.
-
Effect of mutations in the RNA polymerase gene and that of the transcription termination factor rho on expression of the Escherichia coli galactose operon with an IS2 polar insertion.Mol Gen Genet. 1985;201(2):344-6. doi: 10.1007/BF00425682. Mol Gen Genet. 1985. PMID: 3003537
-
[The mechanism of the termination of transcription and the role of rho factor in Escherichia coli (author's transl)].Tanpakushitsu Kakusan Koso. 1977;22(9):1076-90. Tanpakushitsu Kakusan Koso. 1977. PMID: 144931 Review. Japanese. No abstract available.
-
Rho and RNA: models for recognition and response.Mol Microbiol. 1994 Mar;11(6):983-90. doi: 10.1111/j.1365-2958.1994.tb00376.x. Mol Microbiol. 1994. PMID: 8022288 Review.
Cited by
-
Regulation of DNA superhelicity by rpoB mutations that suppress defective Rho-mediated transcription termination in Escherichia coli.J Bacteriol. 1988 Sep;170(9):4266-71. doi: 10.1128/jb.170.9.4266-4271.1988. J Bacteriol. 1988. PMID: 3045090 Free PMC article.
-
Rho-dependent transcription termination of a bacterial operon is antagonized by an extrachromosomal gene product.Proc Natl Acad Sci U S A. 1986 Dec;83(24):9561-5. doi: 10.1073/pnas.83.24.9561. Proc Natl Acad Sci U S A. 1986. PMID: 3540944 Free PMC article.
-
Transcription termination factor rho and T-even phage development.Mol Gen Genet. 1980;177(4):699-705. doi: 10.1007/BF00272682. Mol Gen Genet. 1980. PMID: 6991874
-
Altered transcriptional termination in a rifampicin-resistant mutant of Escherichia coli which inhibits the growth of bacteriophage T7.Mol Gen Genet. 1981;183(1):181-6. doi: 10.1007/BF00270159. Mol Gen Genet. 1981. PMID: 7035818
-
Simultaneous gain and loss of functions caused by a single amino acid substitution in the beta subunit of Escherichia coli RNA polymerase: suppression of nusA and rho mutations and conditional lethality.Genetics. 1992 Mar;130(3):411-28. doi: 10.1093/genetics/130.3.411. Genetics. 1992. PMID: 1551568 Free PMC article.
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
