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. 1978 Jan;75(1):410-4.
doi: 10.1073/pnas.75.1.410.

Quantitative forward mutation assay in Salmonella typhimurium using 8-azaguanine resistance as a genetic marker

T R Skopek, H L Liber, J J Krolewski, W G Thilly

Quantitative forward mutation assay in Salmonella typhimurium using 8-azaguanine resistance as a genetic marker

T R Skopek et al. Proc Natl Acad Sci U S A. 1978 Jan.

Abstract

We have developed a quantitative forward mutation assay using Salmonella typhimurium, in which resistance to the purine analog 8-azaguanine is used as a genetic marker. We present the assay protocol, the concentration-dependent toxicity and mutagenicity of five known mutagens (N-methyl-N'-nitro-N-nitrosoguanidine, ICR-191, 9-aminoacridine, dimethylnitrosamine, and benzo[a]pyrene), and reconstruction experiments testing the assay for possible bias. The relative merits of forward versus reverse mutation assays are discussed.

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