Development of a label free electrochemical sensor based on a sensitive monoclonal antibody for the detection of tiamulin

Abstract

Based on a murine monoclonal antibody (mAb) against tiamulin (TML), an electrochemical immunosensor was proposed using silver-graphene oxide (Ag-GO) nanocomposites and gold nanocomposites (AuNPs) to detect tiamulin (TML). Due to the synergetic properties of Ag-GO nanocomposites and AuNPs, the conductivity of the immunosensor was significantly enhanced. On account of the specific mAb and conductive nanocomposites, the proposed electrochemical immunosensor exhibited a low LOD of 0.003 ng mL^-1 for the detection of TML in a wide linear range of 0.01 to 1000 ng mL^-1. In addition, the immunosensor did not involve additional redox species. Furthermore, the efficient and simple electrochemical immunosensor was employed to detect TML in real samples with high accuracy, suggesting a potential detection platform for other veterinary antibiotics in animal derived foods.

Keywords: AuNPs; Electrochemical immunosensor; Monoclonal antibody; Silver-graphene oxide nanocomposites; Tiamulin.