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. 2021 Jul 1;22(7):2011-2016.
doi: 10.31557/APJCP.2021.22.7.2011.

Molecular and Serological Prevalence of HCMV in Iranian Patients with Breast Cancer

Mohsen Nakhaie  1   2 , Javad Charostad  1   2 , Azarakhsh Azaran  1   2 , Seyyed Ali Mohammad Arabzadeh  3 , Azim Motamedfar  4 , Sara Iranparast  5 , Fatemeh Ahmadpour  6 , Abdolhasan Talaeizadeh  1 , Manoochehr Makvandi  1
Affiliations

Molecular and Serological Prevalence of HCMV in Iranian Patients with Breast Cancer

Mohsen Nakhaie et al. Asian Pac J Cancer Prev. .

Abstract

Background: Human cytomegalovirus (HCMV) is prevalent viral infection involved in several human cancers including breast cancer. The presence of HCMV genome in breast cancer tissue and footprint of viral last exposure patient's serum are considered as important factor in the process of breast cancer development.

Objectives: This study aimed to investigate molecular and serological epidemiology of HCMV in patients with breast cancer in Iran for first time.

Methods: In our case-control study, 98 samples of breast tissue, including 49 cancerous (case) and 49 adjacent non-cancerous tissue were collected (control). In addition, we collected sera samples from all patients (n=49) and healthy individual (n=49). Seroprevalence of HCMV was assessed by Enzyme-linked immunosorbent assay (ELISA) and detection of HCMV genome was performed using Nested-PCR method.

Results: HCMV genome found in 16.3% (8/49) of cases tissue and 2% (1/49) of controls tissue. In patients group, the levels of anti-CMV IgG and IgM were 93.9% and 2% compared to 69.4% and 4.1% in healthy individuals, respectively. There was a statistically difference between the anti-CMV IgG in patients and healthy control (p= 0.002). We found 75% of (6/8) HCMV genome positive PCR samples were also positive for their anti-CMV IgG in cases which was statistically significant (p= 0.01). Conclusions: Our result showed significant presence of HCMV genome and anti-CMV IgG in patients, supporting the role of HCMV in breast cancer.<br />.

Keywords: Breast Neoplasms; Cytomegalovirus; Iran; Prevalence.

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Conflict of interest statement

All authors declared that they have no conflict of interest.

Figures

Figure 1
Figure 1
Positive Polymerase Chain Reaction Bands Corresponding β-Globin (A) and CMV- DNA Polymerase (B)
Figure 2
Figure 2
Percentage of CMV PCR Positivity, Anti-CMV IgG and Anti-CMV IgM in Patients and Healthy Groups
See this image and copyright information in PMC

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