Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2021 Jul 29;19(2):287-296.
doi: 10.1515/jcim-2021-0062. eCollection 2022 Jun 1.

Effect of the beta-adrenergic blockade on intestinal lactate production and glycogen concentration in dogs infused with hexoses

Michael O Allen  1 , Toyin M Salman  2 , Abdul Rasak A Alada  3 , Adeyemi F Odetayo  2 , Eli B Patrick  2 , Shakiru A Salami  1
Affiliations

Effect of the beta-adrenergic blockade on intestinal lactate production and glycogen concentration in dogs infused with hexoses

Michael O Allen et al. J Complement Integr Med. .

Abstract

Objectives: To investigate effect of beta adrenergic blockade on intestinal lactate production and glycogen concentration in dogs infused with hexoses.

Methods: Experiments were carried out on 35 fasted male anaesthetized dogs weighing between 9 and 16 kg. The animals were divided into 7 (5 dogs per group) groups. Group I dogs served as control and infused with normal saline, groups II-IV were intravenously infused with glucose (1.1 mg/kg/min), fructose (1.1 mg/kg/min) and galactose (1.1 mg/kg/min) respectively while groups V-VII animals were pretreated with propranolol (0.5 mg/kg) and were infused with glucose, fructose or galactose respectively. A vein draining the proximal segment of the jejunum was cannulated along with right and left femoral arteries and veins. Glucose uptake was calculated as the product of jejunal blood flow and the difference between arterial and venous glucose levels (A-V glucose), part of the jejunum tissue was homogenized for estimation of glycogen concentration, and plasma lactate was assayed using lactate colorimetric kit.

Results: The result showed significant increase in venous lactate production in response to glucose (78.30 ± 4.57 mg/dL), fructose (60.72 ± 1.82 mg/dL) and galactose (71.70 ± 1.30 mg/dL) when compared with the control group (51.75 ± 1.32 mg/dL) at (p<0.05) with no significant difference in animals pretreated with propranolol. There was no significant difference in glycogen concentration (p>0.05) in animals infused with hexoses only compared with propanolol pretreated group.

Conclusions: Results suggests that one of the possible fates of the enormous amount of glucose taken up by the intestine is conversion to lactate and not glycogen and β-adrenergic receptor does not affect it.

Keywords: beta blockade; glucose uptake; lactate production.

PubMed Disclaimer

References

    1. Grayson, J, Kinnear, T. Observations on temperature, blood flow and heat production in the human liver in relation to environment and to glucose and insulin administration. Clin Sci 1962;22:125–40.
    1. Grayson, J, Oyebola, DD. Effect of catecholamines on intestinal glucose and oxygen uptake in the dog. J Physiol 1983;343:311–22. https://doi.org/10.1113/jphysiol.1983.sp014894.
    1. Oyebola, DD, Durosaiye, GO. Effect of adrenaline and propranolol on glucose uptake in the canine terminal ileum. Niger J Physiol Sci 1988;4:31–7.
    1. Alada, AR, Oyebola, DD. Evidence that the gastrointestinal tract is involved in glucose homeostasis. Afr J Med Med Sci 1996;25:243–9.
    1. Grayson, J, Oyebola, DD. Effect of nicotine on blood flow, oxygen consumption and glucose uptake in the canine small intestine. Br J Pharmacol 1985;85:797–804. https://doi.org/10.1111/j.1476-5381.1985.tb11078.x.

LinkOut - more resources