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. 2021 Jul 29;10(30):e0051921.
doi: 10.1128/MRA.00519-21. Epub 2021 Jul 29.

Genome Sequence of VanLee, a Singleton Actinobacteriophage That Infects Multiple Gordonia Strains

Vanessa Franco  1 , Kaylee Barnhill  1 , Abbigail Biggs  1 , Jackson Bland  1 , Harris Choudhary  1 , Trey Crogan  1 , Alyssa Finocchiaro  1 , Thomas Fuller  1 , Christopher Hanwacker  1 , Zoe Howard  1 , Mohammed Iqbal  1 , Ann Mathew  1 , Sydney Miller  1 , Shivani Padhye  1 , Emily Rainey  1 , Arianna Rodriguez  1 , Emma Stewart  1 , Michael Chase  1 , Louis Otero  1 , Richard S Pollenz  1
Affiliations

Genome Sequence of VanLee, a Singleton Actinobacteriophage That Infects Multiple Gordonia Strains

Vanessa Franco et al. Microbiol Resour Announc. .

Abstract

VanLee is a singleton phage that was isolated from soil in Florida using Gordonia rubripertincta NRRL B-16540 as the host. The genome is 84,560 bp and has a GC content of 67.8%. VanLee has 164 predicted protein-coding genes and one tRNA. VanLee can infect Gordonia terrae with the same efficiency as G. rubripertincta.

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Figures

FIG 1
FIG 1
Transmission electron micrograph of Gordonia phage VanLee (https://phagesdb.org/phages/VanLee). Phage lysates were negatively stained with 1% uranyl acetate. Scale bar = 200 nm.
See this image and copyright information in PMC

References

    1. Watson BNJ, Steens JA, Staals RHJ, Westra ER, van Houte S. 2021. Coevolution between bacterial CRISPR-Cas systems and their bacteriophages. Cell Host Microbe 29:715–725. doi:10.1016/j.chom.2021.03.018. - DOI - PubMed
    1. Jordan TC, Burnett SH, Carson S, Caruso SM, Clase K, DeJong RJ, Dennehy JJ, Denver DR, Dunbar D, Elgin SCR, Findley AM, Gissendanner CR, Golebiewska UP, Guild N, Hartzog GA, Grillo WH, Hollowell GP, Hughes LE, Johnson A, King RA, Lewis LO, Li W, Rosenzweig F, Rubin MR, Saha MS, Sandoz J, Shaffer CD, Taylor B, Temple L, Vazquez E, Ware VC, Barker LP, Bradley KW, Jacobs-Sera D, Pope WH, Russell DA, Cresawn SG, Lopatto D, Bailey CP, Hatfull GF. 2014. A broadly implementable research course in phage discovery and genomics for first-year under-graduate students. mBio 5:e01051-13. doi:10.1128/mBio.01051-13. - DOI - PMC - PubMed
    1. Russell DA, Hatfull GF. 2017. PhagesDB: the Actinobacteriophage Database. Bioinformatics 33:784–786. doi:10.1093/bioinformatics/btw711. - DOI - PMC - PubMed
    1. Silva GG, Dutilh BE, Matthews TD, Elkins K, Schmieder R, Dinsdale EA, Edwards RA. 2013. Combining de novo and reference-guided assembly with Scaffold_builder. Source Code Biol Med 8:23. doi:10.1186/1751-0473-8-23. - DOI - PMC - PubMed
    1. Gordon D, Abajian C, Green P. 1998. Consed: a graphical toll of sequence finishing. Genome Res 8:195–202. doi:10.1101/gr.8.3.195. - DOI - PubMed

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