Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2021 Nov;73(5):590-598.
doi: 10.1111/lam.13544. Epub 2021 Aug 13.

Analysis of antibiotic resistance and virulence patterns in Klebsiella pneumoniae isolated from human urinary tract infections in India

S Bobbadi  1 , B K Chinnam  2 , P N Reddy  1 , S Kandhan  3
Affiliations

Analysis of antibiotic resistance and virulence patterns in Klebsiella pneumoniae isolated from human urinary tract infections in India

S Bobbadi et al. Lett Appl Microbiol. 2021 Nov.

Abstract

In this study, 504 urine samples collected from patients suffering from urinary tract infection (UTIs) were screened for the presence of Klebsiella pneumoniae. The overall occurrence of Klebsiella spp. and K. pneumoniae was found to be 23·2% (117/504) and 16·8% (85/504) respectively. Antibiotic susceptibility testing of 85 K. pneumoniae isolates was carried out by disc diffusion which revealed alarming levels of antibiotic resistance (ABR). Antimicrobial resistance was prominently observed against cefpodoxime (76·47%) followed by ampicillin (70·59%), ceftriaxone (52·94%), cefoxitin (50·59%), amoxyclav (48·24%), ofloxacin (45·88%), cefotaxime (44·71%), cefepime (43·53%) and doxycycline hydrochloride (40%). A small percentage of strains also exhibited resistance to other antimicrobials in the range of 7-35%. Around 77·6% of the isolates were found to be resistant to three or more antibiotic classes and 66·7% of the isolates had multiple antibiotic resistance index values >0·2. Screening of virulence genes in 85 K. pneumoniae isolates revealed that uge gene was the most predominant (11/85, 12·9%), followed by rmpA (9/85, 10·5%), kfu (4/85, 4·7%) and aerobactin genes (2/85, 2·35%). Further, the overall percentage of biofilm producers were found to be 17·65% (15/85). This study warrants hospitals and health care centres to reduce misuse of antibiotics and manage UTI with appropriate treatment after performing antibiotic susceptibility testing.

Keywords: Klebsiella pneumoniae; biofilm; disc diffusion assay; multidrug resistance; urinary tract infections; virulence profile.

PubMed Disclaimer

References

    1. Abadullah, S.M. and Zghair, Z.R. (2016) Isolation of Klebsiella pneumoniae from urine of human and cattle in Baghdad city with histopathological study experimentally in mice. Int J Adv Res Biol Sci 3, 38-45. https://doi.org/10.22192/ijarbs.2016.03.10.006.
    1. Alcántar-Curiel, M.D., Ledezma-Escalante, C.A., Jarillo-Quijada, M.D., Gayosso-Vázquez, C., Morfín-Otero, R., Rodríguez-Noriega, E., Cedillo-Ramírez, M.L., Santos-Preciado, J.I. et al. (2018) Association of antibiotic resistance, cell adherence, and biofilm production with the endemicity of nosocomial Klebsiella pneumoniae. Biomed Res Int 7012958, https://doi.org/10.1155/2018/7012958.
    1. Ali, A.M. and Al-kakei, S. (2019) Virulence factors genotyping of Klebsiella pneumoniae clinical isolates from Baghdad. Rev Med Microbiol 30, 36-46. https://doi.org/10.1097/MRM.0000000000000151.
    1. Bauer, A.W., Kirby, W.M.M., Serris, J.C. and Turck, M. (1966) Antibiotic susceptibility testing by a standardized single disc method. Am J Clin Pathol 45, 149-158.
    1. Bobbadi, S., Chinnam, B.K., Nelapati, S., Tumati, S.R., Kandhan, S., Gottapu, C. and Boddu, S.V. (2019) Occurrence and genetic diversity of ESBL producing Klebsiella species isolated from livestock and livestock products. J Food Saf 40, e12738. https://doi.org/10.1111/jfs.12738.

MeSH terms