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Published Erratum
. 2021 Sep 7;49(15):9000-9001.
doi: 10.1093/nar/gkab625.

Correction to 'Characterization of an antagonistic switch between histone H3 lysine 27 methylation and acetylation in the transcriptional regulation of Polycomb group target genes'

Diego Pasini  1   2 , Martina Malatesta  1   2 , Hye Ryung Jung  2   3 , Julian Walfridsson  1   2 , Anton Willer  1   4 , Linda Olsson  1   2 , Julie Skotte  1   2 , Anton Wutz  5 , Bo Porse  1   4 , Ole Nørregaard Jensen  2   3 , Kristian Helin  1   2
Affiliations
Published Erratum

Correction to 'Characterization of an antagonistic switch between histone H3 lysine 27 methylation and acetylation in the transcriptional regulation of Polycomb group target genes'

Diego Pasini et al. Nucleic Acids Res. .
No abstract available

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Figures

Figure 5.
Figure 5.
p300 and Cbp are required for efficient H3K27 acetylation in Suz12 KO ES cells. (A) qPCR expression analyses of the indicated genes in Suz12 KO ES cells transfected for 48 h with the indicated siRNA oligos. ‘U’ indicates the control siRNA oligo carrying a scrambled oligoribonucleotide sequence. (B) Western blot analyses of histones purified from Suz12 KO ES cells transfected with the indicated siRNA oligos using the indicated antibodies. H3 is presented as loading control. Quantification of the H3/H3K27Ac signal is indicated above each lane. A scrambled siRNA oligo (SCR) was used as negative control. (C and D) Western blot analyses of protein extracts and of purified histones from Suz12 KO ES cells transfected with the indicated siRNA oligos using the indicated antibodies. Vinculin, Ponceau staining and H3 are presented as loading controls. A scrambled siRNA oligo (SCR) was used as negative control. Quantification of the H3/H3K27Ac signal of western blots presented in ‘C’ is indicated above each lane. (E) Average quantification of the H3/H3K27Ac signals between the two independent siRNA experiments presented in C and D.
See this image and copyright information in PMC

Erratum for

References

    1. Pasini D., Malatesta M., Jung H.R., Walfridsson J., Willer A., Olsson L., Skotte J., Wutz A., Porse B., Jensen O.N.et al. .. Characterization of an antagonistic switch between histone H3 lysine 27 methylation and acetylation in the transcriptional regulation of Polycomb group target genes. Nucleic Acids Res. 2010; 38:4958–4969. - PMC - PubMed

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