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. 2021 Sep 15;83(9):1443-1447.
doi: 10.1292/jvms.21-0183. Epub 2021 Jul 31.

Prostaglandins F2α and E2 in rat placenta and fetal membrane: a comprehensive immunohistochemistry of their synthetic enzymes and in vivo tissue levels during normal pregnancy

Hironori Satoh  1 , Ryota Terashima  1 , Mitsumori Kawaminami  1   2 , Shiro Kurusu  1
Affiliations

Prostaglandins F2α and E2 in rat placenta and fetal membrane: a comprehensive immunohistochemistry of their synthetic enzymes and in vivo tissue levels during normal pregnancy

Hironori Satoh et al. J Vet Med Sci. .

Abstract

We determined a comprehensive immunohistochemistry of putative isoforms of enzymes for prostaglandin (PG) F2α and PGE2 biosynthesis and these PGs levels in placenta and fetal membrane of normal pregnant rats in vivo. Placenta and fetal membrane showed positive immunoreactions for phospholipase A2 group 4A, but not group 2A, and cyclooxygenase (COX)-1 rather than COX-2. They showed positive immunoreactions for at least one isoform of each of PGF synthase and PGE synthase with tissue-dependent variations. PGF2α and PGE2 levels in both tissues were highest on day 12 and declined and remained low thereafter. Obtained data would be the basic information on the primary PGs synthesis in rat placenta and fetal membrane in normal pregnancy.

Keywords: fetal membrane; placenta; prostaglandin; rat.

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Conflict of interest statement

We declare no conflict of interest with respect to the publication of this paper.

Figures

Fig. 1.
Fig. 1.
Placental immunoreactivities for prostaglandin F2α and prostaglandin E2 biosynthetic enzymes. Placenta on day 19 of pregnancy was shown with immunostaining of negative control IgG (B), group 2A PLA2(C), group 4A PLA2(A), group 6A PLA2(D), COX-1 (E), COX-2 (F), AKR1C7 (G), AKR1C11 (H), AKR1C5 (I), cPGES (J), mPGES-1 (K) and mPGES-2 (L). White and black arrowheads depicted giant cell and glycogen cell, respectively. BZ, Basal zone; La, Labyrinth. Scale bars, 100 μm in A and 25 μm in B (applicable to C–L).
Fig. 2.
Fig. 2.
Fetal membrane immunoreactivities for prostaglandin F2α and prostaglandin E2 biosynthetic enzymes. Fetal membranes harvested on day 21 of pregnancy were shown with immunostaining of negative control IgG (B), group 2A phospholipase (PL) A2(C), group 4A PLA2(D), group 6A PLA2(E), cyclooxygenase (COX)-1 (A), COX-2 (F), aldo-keto reductase (AKR) 1C7 (G), AKR1C11 (H), AKR1C5 (I), cytosolic type prostaglandin E synthase (cPGES) (J), and microsomal type 1 PGES (mPGES-2) (K) and mPGES-2 (L). Scale bars, 25 μm in A and B (applicable to C–L).
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References

    1. Asami-Miyagishi R., Iseki S., Usui M., Uchida K., Kubo H., Morita I.2004. Expression and function of PPARgamma in rat placental development. Biochem. Biophys. Res. Commun. 315: 497–501. doi: 10.1016/j.bbrc.2004.01.074 - DOI - PubMed
    1. Carminati P., Luzzani F., Soffientini A., Lerner L. J.1975. Influence of day of pregnancy on rat placental, uterine, and ovarian prostaglandin synthesis and metabolism. Endocrinology 97: 1071–1079. doi: 10.1210/endo-97-5-1071 - DOI - PubMed
    1. Chan W. Y.1983. Uterine and placental prostaglandins and their modulation of oxytocin sensitivity and contractility in the parturient uterus. Biol. Reprod. 29: 680–688. doi: 10.1095/biolreprod29.3.680 - DOI - PubMed
    1. Cook J. L., Shallow M. C., Zaragoza D. B., Anderson K. I., Olson D. M.2003. Mouse placental prostaglandins are associated with uterine activation and the timing of birth. Biol. Reprod. 68: 579–587. doi: 10.1095/biolreprod.102.008789 - DOI - PubMed
    1. Freed K. A., Moses E. K., Brennecke S. P., Rice G. E.1997. Differential expression of type II, IV and cytosolic PLA2 messenger RNA in human intrauterine tissues at term. Mol. Hum. Reprod. 3: 493–499. doi: 10.1093/molehr/3.6.493 - DOI - PubMed