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. 2021 Jul 23:2021:2303417.
doi: 10.1155/2021/2303417. eCollection 2021.

Influence of Systemic Administration of Coq10 Nanoparticles on Ischemia-Reperfusion Injury on Ovaries in Rat

Pouria Honardoust  1 , Alireza Najafpour  1 , Rahim Mohammadi  2
Affiliations

Influence of Systemic Administration of Coq10 Nanoparticles on Ischemia-Reperfusion Injury on Ovaries in Rat

Pouria Honardoust et al. Evid Based Complement Alternat Med. .

Abstract

Using a rat ovary model, effects of COQ10 nanoparticles (NCOQ10) were studied on ischemia-reperfusion injury. In the present experimental study, following randomization of thirty healthy female Wistar rats ∼250 g, the animals were subjected to five experimental groups (n = 6): group SHAM : only laparotomy was performed, group IS: only a 3-hour ischemia was performed, group IS/REP: the procedure included a 3-hour ischemia followed by a 3-hour reperfusion, and 50 µL soybean oil (solvent of NCOQ10) was administered 30 min before cessation of reperfusion, group IS/NCOQ10: the procedure included a 3-hour ischemia only and 50 µL (0.3 mmol/lit/IP) of NCOQ10 30 min before cessation of ischemia, and group IS/REP/NCOQ10: the procedure included a 3-hour ischemia, a 3-hour reperfusion, and 20 µL (0.3 mmol/lit) of NCOQ10 30 min before cessation of ischemia. Significantly amended development of ischemia/reperfusion tissue injury was observed in animals treated with NCOQ10 compared to those of other groups (P=0.001). Mean values of biochemical indices were significantly higher than those observed for other groups (P=0.001). Significantly lower values of MDA were observed in IS/REP/NCOQ10 animals compared to those of other groups (P=0.001).Where ovarian tissue is exposed to ischemia, intraperitoneal administration of NCOQ10 could bear clinical benefits in diminishing ischemia-reperfusion injury.

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Conflict of interest statement

There are no conflicts of interest to declare.

Figures

Figure 1
Figure 1
TEM micrograph of nanoparticles.
Figure 2
Figure 2
(a) Size distribution of the nanoparticles based on numbers. (b) Size distribution of the nanoparticles based on volumes.
Figure 3
Figure 3
FTIR spectrum of (a) NCOQ10 and (b) NCOQ10. No significant variation between chemical properties of both particles was observed.
Figure 4
Figure 4
Free NCOQ10 dose response in the reconstitution assay where the acetonitrile was 40%.
Figure 5
Figure 5
Calibration curve of turbidity to identify concentration of nanoparticles.
Figure 6
Figure 6
Representative histologic micrographs of the ovarian samples in SHAM, IS, IS/REP, IS/NCOQ10, and IS/REP/NCOQ10 groups. (a) Normal secondary follicles with compact stroma between them. (b) Many follicles in various stages of development are observed. Edematous ovarian stroma and multiple dilated congested blood vessels with some areas of hemorrhage are observed. (c) Ovarian follicles in various stages of development with edema in the stroma and hemorrhage are observed. (d) Many follicles in various stages of development are observed. There are edematous ovarian stroma and multiple dilated congested blood vessels with some areas of hemorrhage. (e) Normally appearing ovarian tissue with preserved healthy follicles in various stages of development with mild edema or hemorrhage is seen.
Figure 7
Figure 7
Total tissue damage scores of SHAM, IS, IS/REP, IS/NCOQ10, and IS/REP/NCOQ10 groups. Data are expressed as mean ± SD. ∗P < 0.05 vs. IS, IS/REP, and IS/COQ10 groups.
Figure 8
Figure 8
Bar graph depicts comparison of SOD, GPO, GST, and MDA activities in the ovarian samples in experimental groups. Data are expressed as mean ± SD. ∗P < 0.05 vs. IS, IS/REP, and IS/COQ10 groups.
See this image and copyright information in PMC

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