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. 2019 Nov 12;17(4):928-933.
doi: 10.5114/aoms.2019.89692. eCollection 2021.

An investigation of the relation between catalase C262T gene polymorphism and catalase enzyme activity in leukemia patients

Nazan Eras  1 , Gozde Türkoz  2 , Anil Tombak  3 , Naci Tiftik  3 , Serap Yalin  4 , Mehmet Berkoz  5 , Sema Erden  6 , Etem Akbas  2
Affiliations

An investigation of the relation between catalase C262T gene polymorphism and catalase enzyme activity in leukemia patients

Nazan Eras et al. Arch Med Sci. .

Abstract

Introduction: Catalase (CAT), an antioxidant enzyme, catalyzes conversion of hydrogen peroxide to water and molecular oxygen, protecting cells against oxidative stress. The aim of this study was to investigate the possible association between CAT C262T polymorphism in the promoter region of the CAT gene and leukemia risk and to determine the relationship between CAT genotypes and CAT enzyme activities.

Material and methods: Genotypes of 102 cases and 112 healthy controls' genotypes were determined by polymerase chain reaction-restriction fragment length polymorphism methods. Catalase activity was measured with the method of Aebi.

Results: The frequencies of the T allele among the cases and controls were 28.4% and 25.9%, respectively (p = 0.75). The frequencies of CC, CT, and TT among cases were 57.8%, 27.4%, and 14.7%, respectively, while in controls, the frequencies of CC, CT, and TT were 54.4%, 39.3%, and 6.3%, respectively, which were not significantly different. Although CAT enzyme activity was lower in leukemia patients with TT genotypes than in controls, this did not reach statistical significance (p = 0.37).

Conclusions: This is the first report showing that CAT C262T polymorphism is not a genetic predisposing factor for the risk of leukemia in the Turkish population. However, additional research is needed to confirm these findings.

Keywords: antioxidant; catalase; leukemia; oxidative stress; polymorphism.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
CAT-262 C/T genotyping by PCR-RFLP. M: 100-bp ladder; Lanes 1, 2, 4: C/C (155 bp); 3: T/T (185 bp); 5: C/T (185, 155 bp)
See this image and copyright information in PMC

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