Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 Mar;80(1):89-95.
doi: 10.1007/s12013-021-01025-5. Epub 2021 Aug 3.

INitial Steps of Insulin Action in Parotid Glands of Male Wistar Rats

André Guaraci DeVito-Moraes  1   2   3 , Victor Di Donato Marques  4 , Luciana Chagas Caperuto  4   5 , Flavia Kazue Ibuki  6 , Fernando Neves Nogueira  6 , Carlos Eduardo Francci  6 , Carla Roberta de Oliveira Carvalho  4
Affiliations

INitial Steps of Insulin Action in Parotid Glands of Male Wistar Rats

André Guaraci DeVito-Moraes et al. Cell Biochem Biophys. 2022 Mar.

Abstract

The parotid gland is the largest salivary gland. It produces watery saliva, rich in proteins (amylase, lysozymes, and antibodies). Due to the gland's morphological cytoarchitecture composed of only serous acini, it contributes almost 50% of total salivary volume upon stimulation. It has been reported that the prevalence of saliva secretion impairments, periodontitis, delayed wound healing, and xerostomia increase in diabetic patients. Herein we evaluated the acute effects of insulin on insulin receptor phosphorylation status and its substrates IRS-1 and IRS-2 in the parotid glands of adult male Wistar rats, using Western blot analyses. We confirmed an acute effect of insulin on IR/IRS/PI3K/Akt and MAPK intracellular pathway activation in the parotid glands of male Wistar rats similar to the classical metabolic targets of the hormone, like the liver.

Keywords: Insulin; Intracellular Signaling, Rats.; Parotid Gland.

PubMed Disclaimer

References

    1. White, M. F., Shoelson, S. E., Keutmann, H., & Kahn, C. R. (1988). A cascade of tyrosine autophosphorylation in the beta-subunit activates the phosphotransferase of the insulin receptor. Journal of Biological Chemistry, 263(6), 2969–2980.
    1. Cheatham, B., & Kahn, C. R. (1995). Insulin action and the insulin-signaling network. Endocrine Review, 16, 117–142.
    1. Cuatrecasas, P. (1969). Interaction of insulin with the cell membrane: the primary action of insulin. Biochem, 63, 450–457.
    1. Cuatrecasas, P. (1972). Affinity chromatography and purification of the insulin receptor of liver cell membranes. Proceedings of the National Academy of Sciences of the United States of America, 69, 1277–1281. - PubMed - PMC
    1. Freychet, P., Roth, J., & Neville, D. M. (1971). Monoiodoinsulin: demonstration of its biological activity and binding to fat cells and liver membranes. Biochemical and Biophysical Research Communications, 43(2), 400–408. - PubMed

LinkOut - more resources