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. 2021 Nov;15(6):707-710.
doi: 10.1111/irv.12892. Epub 2021 Aug 3.

A Sanger sequencing protocol for SARS-CoV-2 S-gene

Rodney S Daniels  1 , Ruth Harvey  1 , Burcu Ermetal  1 , Zheng Xiang  1 , Monica Galiano  1 , Lorin Adams  1 , John W McCauley  1
Affiliations

A Sanger sequencing protocol for SARS-CoV-2 S-gene

Rodney S Daniels et al. Influenza Other Respir Viruses. 2021 Nov.

Abstract

We describe a Sanger sequencing protocol for SARS-CoV-2 S-gene the Spike (S)-glycoprotein product of which, composed of receptor-binding (S1) and membrane fusion (S2) segments, is the target of vaccines used to combat COVID-19. The protocol can be used in laboratories with basic Sanger sequencing capabilities and allows rapid "at source" screening for SARS-CoV-2 variants, notably those of concern. The protocol has been applied for surveillance, with clinical specimens collected in either nucleic acid preservation lysis-mix or virus transport medium, and research involving cultured viruses, and can yield data of public health importance in a timely manner.

Keywords: S-gene; SARS-CoV-2; Sanger sequencing; base-calling accuracy; variant detection.

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Conflict of interest statement

All authors declare no conflicts of interest.

References

    1. World Health Organization . Operational considerations for COVID‐19 surveillance using GISRS: interim guidance 2020. https://apps.who.int/iris/bitstream/handle/10665/331589/WHO-2019-nCoV-Le... Accessed July 14, 2021.
    1. World Health Organization . Preparing GISRS for the upcoming influenza seasons during the COVID‐19 pandemic—practical consideration 2020. https://apps.who.int/iris/bitstream/handle/10665/332198/WHO-2019-nCoV-Pr... Accessed July 14, 2021.
    1. Wise J. COVID‐19: new coronavirus variant is identified in UK. BMJ. 2020;37:m4857. - PubMed
    1. Tegally H, Wilkinson E, Giovanetti M, et al. Detection of a SARS‐CoV‐2 variant of concern in South Africa. Nature. 2021;592:438‐443. - PubMed
    1. Garcia‐Beltran WF, Lam EC, St Denis K, et al. Multiple SARS‐CoV‐2 variants escape neutralization by vaccine‐induced humoral immunity. Cell. 2021;184(9):2372‐2383. - PMC - PubMed

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