Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2021 Sep 20;59(10):e0094621.
doi: 10.1128/JCM.00946-21. Epub 2021 Aug 4.

Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Rapid Detection of Isolates Belonging to the Epidemic Clones Achromobacter xylosoxidans ST137 and Achromobacter ruhlandii DES from Cystic Fibrosis Patients

Thomas Garrigos  1   2 , Manon Dollat  1 , Arnaud Magallon  1   2 , Angélique Chapuis  1 , Véronique Varin  1 , Julien Bador  1   2 , Nadia Makki  3 , Lise Cremet  4 , Elise Persyn  4 , Emilie Cardot-Martin  5 , Fedoua Echahidi  6 , Charlotte Peeters  7 , Denis Pierard  6 , Peter Vandamme  7 , Alexia Verroken  8 , Catherine Neuwirth  1   2 , Lucie Amoureux  1   2
Affiliations

Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Rapid Detection of Isolates Belonging to the Epidemic Clones Achromobacter xylosoxidans ST137 and Achromobacter ruhlandii DES from Cystic Fibrosis Patients

Thomas Garrigos et al. J Clin Microbiol. .

Abstract

Achromobacter spp. are increasingly reported among cystic fibrosis patients. Genotyping requires time-consuming methods such as multilocus sequence typing or pulsed-field gel electrophoresis. Therefore, data on the prevalence of multiresistant epidemic clones, especially A. xylosoxidans ST137 (AxST137) and the Danish epidemic strain A. ruhlandii (DES), are lacking. We recently developed and published a database for Achromobacter species identification by matrix-assisted laser desorption-ionization-time of flight mass spectrometry (MALDI-TOF MS; Bruker Daltonics). The aim of this study was to evaluate the ability of the MALDI-TOF MS to distinguish these multiresistant epidemic clones within Achromobacter species. All the spectra of A. xylosoxidans (n = 1,571) and A. ruhlandii (n = 174) used to build the local database were analyzed by ClinProTools, MALDI Biotyper PCA, MALDI Biotyper dendrogram, and flexAnalysis software for biomarker peak detection. Two hundred two isolates (including 48 isolates of AxST137 and 7 of DES) were tested. Specific biomarker peaks were identified: absent peak at m/z 6,651 for AxST137 isolates and present peak at m/z 9,438 for DES isolates. All tested isolates were well typed by our local database and clustered within distinct groups (ST137 or non-ST137 and DES or non-DES) no matter the MALDI-TOF software or only by simple visual inspection of the spectra by any user. The use of MALDI-TOF MS allowed us to identify isolates of A. xylosoxidans belonging to the AxST137 clone that spread in France and Belgium (the Belgian epidemic clone) and of A. ruhlandii belonging to the DES clone. This tool will help the implementation of segregation measures to avoid interpatient transmission of these resistant clones.

Keywords: Achromobacter ruhlandii; Achromobacter xylosoxidans; Danish epidemic strain; MALDI-TOF MS; ST137; cystic fibrosis; epidemic clones; typing.

PubMed Disclaimer

Figures

FIG 1
FIG 1
Representation of clusters built by PCA on MALDI Biotyper software and biomarker peaks: absence of a peak at m/z 6,651 (6,640 to 6,700) for A. xylosoxidans ST137 and presence of a peak at m/z 9,438 for A. ruhlandii DES using flexAnalysis software.
FIG 2
FIG 2
MSP dendrogram built with A. xylosoxidans isolates belonging to different STs using MALDI Biotyper software with MSP dendrogram creation standard method, with default settings.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Spilker T, Vandamme P, Lipuma JJ. 2013. Identification and distribution of Achromobacter species in cystic fibrosis. J Cyst Fibros 12:298–301. 10.1016/j.jcf.2012.10.002. - DOI - PubMed
    1. Papalia M, Steffanowski C, Traglia G, Almuzara M, Martina P, Galanternik L, Vay C, Gutkind G, Ramírez MS, Radice M. 2020. Diversity of Achromobacter species recovered from patients with cystic fibrosis, in Argentina. Rev Argent Microbiol 52:13–18. 10.1016/j.ram.2019.03.004. - DOI - PubMed
    1. Gade SS, Nørskov-Lauritsen N, Ridderberg W. 2017. Prevalence and species distribution of Achromobacter sp. cultured from cystic fibrosis patients attending the Aarhus Centre in Denmark. J Med Microbiol 66:686–689. 10.1099/jmm.0.000499. - DOI - PubMed
    1. Pereira RHV, Leão RS, Carvalho-Assef AP, Albano RM, Rodrigues ERA, Firmida MC, Folescu TW, Plotkowski MC, Bernardo VG, Marques EA. 2017. Patterns of virulence factor expression and antimicrobial resistance in Achromobacter xylosoxidans and Achromobacter ruhlandii isolates from patients with cystic fibrosis. Epidemiol Infect 145:600–606. 10.1017/S0950268816002624. - DOI - PMC - PubMed
    1. Hansen CR, Pressler T, Nielsen KG, Jensen PØ, Bjarnsholt T, Høiby N. 2010. Inflammation in Achromobacter xylosoxidans infected cystic fibrosis patients. J Cyst Fibros off J Eur Cyst Fibros Soc 9:51–58. 10.1016/j.jcf.2009.10.005. - DOI - PubMed

MeSH terms

Supplementary concepts