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Comment
. 2021 Aug 17;120(16):3237-3239.
doi: 10.1016/j.bpj.2021.07.005. Epub 2021 Jul 9.

Extending the performance capabilities of isoSTED

Ulrike Boehm  1 , Catherine G Galbraith  2
Affiliations
Comment

Extending the performance capabilities of isoSTED

Ulrike Boehm et al. Biophys J. .
No abstract available

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Figures

Figure 1
Figure 1
How the effective excitation profile contributes to OOFCs in thick specimens. (A) Intensity profile of the effective excitation (solid line) along the optical (z axis) shows the magnitude of PSF side lobes (OOFCs) in comparison to the central excitation point; all are collected by the diffraction-limited detection (dashed line). (B) In a thin region of the cell, only the main focus (small circle) contributes to the detected signal, whereas, in a thick region of the cell, OOFCs (large oval) contribute to the signal and can be detected by a laterally shifted detector for subsequent removal (dashed microtubules). Modified from (6). To see this figure in color, go online.
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References

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